Selection of Salmonella enterica serovar Typhi genes involved during interaction with human macrophages by screening of a transposon mutant library.

PloS One
Sébastien C SabbaghFrance Daigle

Abstract

The human-adapted Salmonella enterica serovar Typhi (S. Typhi) causes a systemic infection known as typhoid fever. This disease relies on the ability of the bacterium to survive within macrophages. In order to identify genes involved during interaction with macrophages, a pool of approximately 10(5) transposon mutants of S. Typhi was subjected to three serial passages of 24 hours through human macrophages. Mutants recovered from infected macrophages (output) were compared to the initial pool (input) and those significantly underrepresented resulted in the identification of 130 genes encoding for cell membrane components, fimbriae, flagella, regulatory processes, pathogenesis, and many genes of unknown function. Defined deletions in 28 genes or gene clusters were created and mutants were evaluated in competitive and individual infection assays for uptake and intracellular survival during interaction with human macrophages. Overall, 26 mutants had defects in the competitive assay and 14 mutants had defects in the individual assay. Twelve mutants had defects in both assays, including acrA, exbDB, flhCD, fliC, gppA, mlc, pgtE, typA, waaQGP, SPI-4, STY1867-68, and STY2346. The complementation of several mutants by expression of plas...Continue Reading

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Citations

Sep 11, 2014·Annual Review of Microbiology·Gordon Dougan, Stephen Baker
Oct 9, 2015·The Journal of Antimicrobial Chemotherapy·Clara Ballesté-DelpierreJordi Vila
Oct 30, 2012·Cell·Ferric C Fang, Andreas J Bäumler
Nov 13, 2015·PLoS Pathogens·Shabarinath SrikumarJay C D Hinton
Feb 24, 2018·Frontiers in Cellular and Infection Microbiology·Karine DufresneFrance Daigle
Jul 22, 2018·Cellular Microbiology·Rebecca JohnsonGad Frankel
Feb 6, 2020·Infection and Immunity·Daniel HurleySéamus Fanning
Dec 2, 2015·The Biochemical Journal·Vincenzo CrescenteAli Ryan
Dec 2, 2020·Infection and Immunity·Samantha D CraneRoger D Pechous
Sep 16, 2019·Microbial Pathogenesis·Tayyab RehmanPing Ouyang

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Methods Mentioned

BETA
PCR

Software Mentioned

GenePix Pro

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