Selection of the initiator tRNA by Escherichia coli initiation factors
We have developed a new technique, called 'toeprinting,' which has allowed a study of the tRNA-binding properties of Escherichia coli translation initiation complexes. In response to natural mRNAs, the initiator tRNA and a variety of elongator tRNAs bind to the same tRNA-binding site on the 30S ribosomal subunit as long as a cognate codon is present near the Shine and Dalgarno sequence. The selection of the initiator tRNA in 30S initiation complexes is accomplished by initiation factors IF2 and IF3. 70S ribosomes accept both initiator tRNA and elongator tRNAs on natural mRNAs, much like 30S ribosomal subunits; IF3 and IF2 do not, however, select the initiator tRNA on 70S initiation complexes unless the initiation factor IF1 is present.
Ribosome-binding sites on chloroplast rbcL and psbA mRNAs and light-induced initiation of D1 translation
A proposed role for IF-3 and EF-T in maintaining the specificity of prokaryotic initiation complex formation
Translational regulation of the Escherichia coli threonyl-tRNA synthetase gene: structural and functional importance of the thrS operator domains
Differential inhibition of 30S and 70S translation initiation complexes on leaderless mRNA by kasugamycin
Initiation of Escherichia coli ribosomes on matrix coupled mRNAs studied by optical biosensor technique
Recognition of the initiator tRNA by the Pseudomonas aeruginosa methionyl-tRNA formyltransferase: importance of the base-base mismatch at the end of the acceptor stem
Toeprint analysis of the positioning of translation apparatus components at initiation and termination codons of fungal mRNAs
Identification of the ribosome binding sites of translation initiation factor IF3 by multidimensional heteronuclear NMR spectroscopy
Mapping the active site of the Haemophilus influenzae methionyl-tRNA formyltransferase: residues important for catalysis and tRNA binding
Initiation factors of protein biosynthesis in bacteria and their structural relationship to elongation and termination factors
Translation of mRNAs with degenerate initiation triplet AUU displays high initiation factor 2 dependence and is subject to initiation factor 3 repression
Selective stimulation of translation of leaderless mRNA by initiation factor 2: evolutionary implications for translation
Molecular mimicry in translational control of E. coli threonyl-tRNA synthetase gene. Competitive inhibition in tRNA aminoacylation and operator-repressor recognition switch using tRNA identity rules
Interaction of the Escherichia coli fdhF mRNA hairpin promoting selenocysteine incorporation with the ribosome
Role of the N- and C-terminal extensions on the activity of mammalian mitochondrial translational initiation factor 3
Both RNase E and RNase III control the stability of sodB mRNA upon translational inhibition by the small regulatory RNA RyhB
Crucial contribution of the multiple copies of the initiator tRNA genes in the fidelity of tRNA(fMet) selection on the ribosomal P-site in Escherichia coli
The roles of individual eukaryotic translation initiation factors in ribosomal scanning and initiation codon selection
Position of eukaryotic initiation factor eIF1 on the 40S ribosomal subunit determined by directed hydroxyl radical probing
Molecular characterisation of the pifC gene encoding translation initiation factor 3, which is required for normal photosynthetic complex formation in Rhodobacter sphaeroides NCIB 8253
The sarcin-ricin loop of 23S rRNA is essential for assembly of the functional core of the 50S ribosomal subunit
Initiation factor-independent translation mediated by the hepatitis C virus internal ribosome entry site
Concerted actions of a thermo-labile regulator and a unique intergenic RNA thermosensor control Yersinia virulence
Divergent tRNA-like element supports initiation, elongation, and termination of protein biosynthesis
Identification of molecular interactions between P-site tRNA and the ribosome essential for translocation
Taura syndrome virus IRES initiates translation by binding its tRNA-mRNA-like structural element in the ribosomal decoding center
70S-scanning initiation is a novel and frequent initiation mode of ribosomal translation in bacteria
Ribosomal initiation complex-driven changes in the stability and dynamics of initiation factor 2 regulate the fidelity of translation initiation
Translation initiation factor 3 regulates switching between different modes of ribosomal subunit joining
The influence of ribosome-binding-site elements on translational efficiency in Bacillus subtilis and Escherichia coli in vivo
Ribosomal intersubunit bridge B2a is involved in factor-dependent translation initiation and translational processivity
A single mutation in the IF3 N-terminal domain perturbs the fidelity of translation initiation at three levels
Overexpression of RbfA in the absence of the KsgA checkpoint results in impaired translation initiation
Nucleotides of tRNA governing the specificity of Escherichia coli methionyl-tRNA(fMet) formyltransferase
Detection of Escherichia coli ribosome binding at translation initiation sites in the absence of tRNA
Non-bridging phosphate oxygen atoms within the tRNA anticodon stem-loop are essential for ribosomal A site binding and translocation
Translation initiation in the crenarchaeon Sulfolobus solfataricus: eukaryotic features but bacterial route
Multiple ways to regulate translation initiation in bacteria: Mechanisms, regulatory circuits, dynamics
The emerging role of rectified thermal fluctuations in initiator aa-tRNA- and start codon selection during translation initiation
The 5'-7-methylguanosine cap on eukaryotic mRNAs serves both to stimulate canonical translation initiation and to block an alternative pathway
How initiation factors maximize the accuracy of tRNA selection in initiation of bacterial protein synthesis
Universally conserved interactions between the ribosome and the anticodon stem-loop of A site tRNA important for translocation
Genetically encoded but nonpolypeptide prolyl-tRNA functions in the A site for SecM-mediated ribosomal stall
Accurate translocation of mRNA by the ribosome requires a peptidyl group or its analog on the tRNA moving into the 30S P site
Dual translational start motif evolutionarily conserved in the holin gene of Bacillus subtilis phage phi 29
Staphylococcus aureus RNAIII binds to two distant regions of coa mRNA to arrest translation and promote mRNA degradation
A Staphylococcus aureus small RNA is required for bacterial virulence and regulates the expression of an immune-evasion molecule
5'-Terminal AUGs in Escherichia coli mRNAs with Shine-Dalgarno Sequences: Identification and Analysis of Their Roles in Non-Canonical Translation Initiation
The conserved A-site finger of the 23S rRNA: just one of the intersubunit bridges or a part of the allosteric communication pathway?
Nascent peptide in the ribosome exit tunnel affects functional properties of the A-site of the peptidyl transferase center
A new model for the three-dimensional folding of Escherichia coli 16 S ribosomal RNA. III. The topography of the functional centre
Escherichia coli initiation factor 3 protein binding to 30S ribosomal subunits alters the accessibility of nucleotides within the conserved central region of 16S rRNA
Structure-function analysis of Escherichia coli translation initiation factor IF3: tyrosine 107 and lysine 110 are required for ribosome binding
Evidence for processivity and two-step binding of the RNA substrate from studies of J1/2 mutants of the Tetrahymena ribozyme
Nature of the ribosomal mRNA track: analysis of ribosome-binding sites containing different sequences and secondary structures
Initiation factor 3-induced structural changes in the 30 S ribosomal subunit and in complexes containing tRNA(f)(Met) and mRNA
Prokaryotic translation initiation factor IF3 is an elongated protein consisting of two crystallizable domains
A conformational switch in initiation factor 2 controls the fidelity of translation initiation in bacteria
Codon-anticodon interaction at the P site is a prerequisite for tRNA interaction with the small ribosomal subunit
Identification of mammalian mitochondrial translational initiation factor 3 and examination of its role in initiation complex formation with natural mRNAs.
Discrimination of 5'-terminal start codons by translation initiation factor 3 is mediated by ribosomal protein S1
Direct monitoring of initiation factor dynamics through formation of 30S and 70S translation-initiation complexes on a quartz crystal microbalance
Contributions of the N- and C-Terminal Domains of Initiation Factor 3 to Its Functions in the Fidelity of Initiation and Antiassociation of the Ribosomal Subunits
Coevolution of the translational machinery optimizes initiation with unusual initiator tRNAs and initiation codons in mycoplasmas
A pseudoknot is required for efficient translational initiation and regulation of the Escherichia coli rpsO gene coding for ribosomal protein S15
Mutants of Escherichia coli initiator tRNA defective in initiation. Effects of overproduction of methionyl-tRNA transformylase and the initiation factors IF2 and IF3.
Regulation of the activity of chloroplast translational initiation factor 3 by NH2- and COOH-terminal extensions.
Evidence for the translation initiation of leaderless mRNAs by the intact 70 S ribosome without its dissociation into subunits in eubacteria.
Interaction of fMet-tRNAfMet and fMet-AMP with the C-terminal domain of Thermus thermophilus translation initiation factor 2
Escherichia coli initiator tRNA: structure-function relationships and interactions with the translational machinery
The Pseudomonas aeruginosa initiation factor IF-2 is responsible for formylation-independent protein initiation in P. aeruginosa.
Rapid formylation of the cellular initiator tRNA population makes a crucial contribution to its exclusive participation at the step of initiation
Structural basis of sequestration of the anti-Shine-Dalgarno sequence in the Bacteroidetes ribosome.
Search for translation arrest peptides encoded upstream of genes for components of protein localization pathways.
Identification of an intragenic ribosome binding site that affects expression of the uncB gene of the Escherichia coli proton-translocating ATPase (unc) operon
Heteronuclear NMR studies of E. coli translation initiation factor IF3. Evidence that the inter-domain region is disordered in solution
Discrimination by Escherichia coli initiation factor IF3 against initiation on non-canonical codons relies on complementarity rules
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