Selection of the T cell repertoire during ontogeny: limiting dilution analysis

European Journal of Immunology
H S TehH Von Boehmer

Abstract

The relative frequencies of cytotoxic T lymphocyte precursors (CTL-P) specific for minor histocompatibility antigens that are restricted to either k or b major histocompatibility complex antigens in normal B6 mice and in B6 leads to (B6 x CBA)F1 radiation chimeras were estimated. The ratio of k-restricted to b-restricted CTL-P determined for the radiation chimeras served as a base value to which the ratio of k: b-restricted CTL from normal B6 mice can be compared. The frequencies of allorestricted CTL-P in B6 mice were determined after the B6 cells had been depleted of k-reactive cells by filtration through irradiated B6.H-2k mice. Lymphocytes from immunized animals were used to obtain frequency estimates of CTL-P specific for minor H antigens. The cultures contained nu/nu spleen cells syngeneic to the responder, but no exogenous interleukin 2 (T cell growth factor) since a high degree of nonspecific killing was found in IL2-supplemented cultures. It was found that the CTL-P frequency of B6 mice to BALB.B antigens in the lymph node cells of normal or immune B6 mice were 1 in 300 000 and 1 in 8000, respectively. In B6 leads to (B6 x CBA)F1 radiation chimeras, the ratio of BALB.B/BALB.K-specific CTL-P was 3:1; this ratio was 17:1...Continue Reading

References

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Citations

Sep 24, 1999·Immunologic Research·I N Crispe
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M F Good, G J Nossal
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·K WietiesJ Forman
Mar 1, 1991·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·A Juretic, B B Knowles
Jul 1, 1983·Annales d'immunologie·H von BoehmerW Haas
Oct 1, 1993·Immunological Reviews·H von BoehmerP Kisielow
Jul 1, 1984·The American Journal of Anatomy·J J Owen, E J Jenkinson
Nov 1, 1984·European Journal of Immunology·W HaasH Von Boehmer
May 1, 2009·Journal of Immunotoxicology·Dmitry B Kazansky
Sep 9, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jean KwunStuart J Knechtle
Apr 1, 1985·European Journal of Immunology·J ReimannH Wagner
Mar 1, 1983·European Journal of Immunology·H S TehH Von Boehmer
Apr 1, 1985·European Journal of Immunology·E L LarssonM Ermonval
Feb 1, 1989·Immunology Today·H von BoehmerP Kisielow

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