PMID: 6409969Aug 1, 1983Paper

Selective aberration and pigment loss in melanosomes of malignant melanoma cells in vitro by glycosylation inhibitors: premelanosomes as glycoprotein

The Journal of Investigative Dermatology
Y Mishima, G Imokawa

Abstract

We have found that glucosamine (1 mg/ml) or tunicamycin (0.2-0.4 micrograms/ml), specific inhibitors of lipid carrier-dependent glycosylation of protein, when added to cultured B-16 melanoma cells produce a marked loss of pigmentation, accompanied by distinctive biochemical as well as ultrastructural aberrations in their melanogenic compartments. Electron microscopic analysis shows that these newly induced unpigmented cells form uniquely altered melanosomes containing little or no melanin, although their population is not substantially reduced. Within the melanogenic compartments, selective aberration of melanosomes is seen, that is, deformity, bulging, and segregation of their interior membrane, as well as the intramelanosomal formation of irregularly concentric lamellar structure. No apparent structural deformity of Golgi apparatus, Golgi-associated endoplasmic reticulum of lysosome (GERL), and coated vesicles has been observed. Further, no substantial alteration is seen in mitochondria or other cellular structures. Quantitative analysis of altered and nonaltered melanosomes has revealed that the ratio of altered premelanosomes to the total number increases to 44% in glucosamine-treated cells and to 99.5% in tunicamycin-treat...Continue Reading

References

May 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A KaplanW S Sly
Nov 1, 1979·European Journal of Biochemistry·K von FiguraK Ullrich
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Citations

Jun 17, 2003·Biological & Pharmaceutical Bulletin·Sook-Jung ImWon-Hong Woo
Dec 1, 2007·Journal of Cosmetic Dermatology·Donald L BissettMichael K Robinson
Oct 1, 1990·Pigment Cell Research·H AndoY Mishima
Jan 1, 1985·Archives of Dermatological Research·B SahlmannP Stanka
Jan 1, 1992·Pigment Cell Research·S J OrlowJ M Pawelek
Feb 21, 2002·Biological & Pharmaceutical Bulletin·Akemi KobayashiYoshinori Takema
Jan 20, 2006·Pigment Cell Research·Ning Wang, Daniel N Hebert
Mar 1, 2012·Annals of Dermatology·Hyojin KimKyoung-Chan Park
May 19, 2020·Nucleic Acids Research·Pascal StücheliMartin Fussenegger
Mar 8, 2003·Pigment Cell Research·Stefania BrigantiMauro Picardo
Mar 14, 2007·Journal of Cosmetic Dermatology·Donald L BissettGary R Kelm
Dec 1, 1988·Journal of Cutaneous Pathology·F O'ValleT Alvaro
Oct 30, 2009·International Journal of Molecular Sciences·Jody P EbanksRaymond E Boissy
Aug 1, 1988·The Journal of Investigative Dermatology·S HattaS Ito
Nov 1, 1989·The Journal of Investigative Dermatology·A K ChakrabortyM Ichihashi
Feb 2, 2008·The Journal of Investigative Dermatology·Shinya KasamatsuRaymond E Boissy

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