Selective ablation of TRPV1 by intrathecal injection of resiniferatoxin in rats increases renal sympathoexcitatory responses and salt sensitivity

Hypertension Research : Official Journal of the Japanese Society of Hypertension
Shuang-Quan YuDonna H Wang

Abstract

This study tested the hypothesis that selective ablation of transient receptor potential vanilloid type 1 (TRPV1)-positive nerve fibers by intrathecal injection of resiniferatoxin (RTX) enhances renal sympathoexcitatory responses and salt sensitivity. Intrathecal injection of RTX (1.8 μg/kg) to the levels of lower thoracic and upper lumbar spinal cord (T8-L3) increased mean arterial pressure (MAP) in rats fed a normal (NS, 1% NaCl) or high-sodium (HS, 8% NaCl) diet for 4 weeks compared to vehicle-treated rats (NS: 121 ± 2 vs. 111 ± 2; HS: 154 ± 2 vs. 134 ± 2 mm Hg, both P < 0.05), with a greater increase in HS compared to NS rats (9 ± 1% vs. 15 ± 1%, P < 0.05). TRPV1 contents were decreased in T8-L3 segments of spinal dorsal horn but not in corresponding dorsal root ganglia and the kidney following RTX treatment (P < 0.05). Selective activation of GABA-A receptors with intrathecal T8-L3 segment-injection of muscimol (3 nmol/kg) decreased renal sympathetic nerve activity and increased urinary excretion in both NS and HS rats, with a greater effect in RTX-treated compared to vehicle-treated rats (P < 0.05). Chronic activation of GABA-A receptors with muscimol (50 mg/kg/day × 2, p.o.) abolished RTX treatment-induced pressor effect...Continue Reading

References

Jun 25, 1976·Science·T L Yaksh, T A Rudy
Dec 15, 1994·Journal of the Autonomic Nervous System·M BurgM M Knuepfer
Dec 1, 1996·The European Journal of Neuroscience·A DumoulinS Feldblum
Oct 23, 2001·American Journal of Physiology. Heart and Circulatory Physiology·Y Huang, D H Wang
May 16, 2002·Clinical and Experimental Pharmacology & Physiology·Ida J Llewellyn-Smith
Feb 6, 2003·Journal of Hypertension·Donna H Wang, Yajuan Zhao
Mar 18, 2003·Journal of Hypertension·Jianping Li, Donna H Wang
Jul 23, 2003·Journal of Hypertension·Jianping Li, Donna H Wang
Feb 18, 2005·Acta Pharmacologica Sinica·Donna H Wang
Jul 23, 2005·The Journal of Physiology·Manish RaisinghaniLouis S Premkumar
May 25, 2006·Naunyn-Schmiedeberg's Archives of Pharmacology·António Avelino, Francisco Cruz
Jun 17, 2006·Annual Review of Neuroscience·Ajay DhakaArdem Patapoutian
Aug 22, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Youping WangDonna H Wang
Aug 19, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ulla C KoppTomas Hökfelt
Oct 24, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ulla C KoppGerald F DiBona
Nov 18, 2008·Journal of Neurochemistry·Hong-Yi ZhouHui-Lin Pan
Nov 21, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lihua WangSusan A Deuchars
Jun 7, 2011·Acta Pharmacologica Sinica·Shuang-quan Yu, Donna H Wang
Nov 21, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ulla C Kopp
Dec 4, 2015·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Heitaro WatanabeShunichi Miyazaki
Dec 15, 2015·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jason D FossJohn W Osborn
Feb 13, 2016·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Domenico Di RaimondoAntonio Pinto
Feb 26, 2016·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Pavel SvitokMichal Zeman

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
urine collection
Assay

Software Mentioned

ImageJ
P3 Plus
Olympus MicroSuiteTM - Basic

Related Concepts

Related Feeds

Antihypertensive Agents: Mechanisms of Action

Antihypertensive drugs are used to treat hypertension (high blood pressure) which aims to prevent the complications of high blood pressure, such as stroke and myocardial infarction. Discover the latest research on antihypertensive drugs and their mechanism of action here.