PMID: 7528142Dec 1, 1994Paper

Selective activation and inhibition of calmodulin-dependent enzymes by a calmodulin-like protein found in human epithelial cells

European Journal of Biochemistry
C F EdmanP Yaswen

Abstract

A calmodulin-like protein, which is identical in size and 85% identical to vertebrate calmodulin, was recently identified by 'subtractive hybridization' comparison of transcripts expressed in normal versus transformed human mammary epithelial cells. Unlike the ubiquitous distribution of calmodulin, calmodulin-like protein expression is restricted to certain epithelial cells, and appears to be modulated during differentiation. In addition, calmodulin-like protein levels are often significantly reduced in malignant tumor cells as compared to corresponding normal epithelial cells. The current studies compare calmodulin-like protein functions with those of calmodulin. We find that calmodulin-like protein activation of multifunctional Ca2+/calmodulin-dependent protein kinase II (calmodulin kinase II) is equivalent to activation by calmodulin, but that four other calmodulin-dependent enzymes, cGMP phosphodiesterase, calcineurin, nitric-oxide synthase, and myosin-light-chain kinase, display much weaker activation by calmodulin-like protein than by calmodulin. In the case of myosin-light-chain kinase, calmodulin-like protein competitively inhibits calmodulin activation of the enzyme with a Ki value of 170 nM. Thus, calmodulin-like prot...Continue Reading

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Citations

Jan 1, 1995·Virus Genes·J Brosius, H Tiedge
Aug 10, 2000·Neoplasia : an International Journal for Oncology Research·M S RogersE E Strehler
Jan 27, 2009·Journal of Prosthodontics : Official Journal of the American College of Prosthodontists·Michael D BrooksAlan B Carr
Dec 14, 2006·Journal of Cellular Biochemistry·Ji-Yeon BaeDong-Young Noh
Mar 30, 2001·The Journal of Biological Chemistry·M S Rogers, E E Strehler
Aug 20, 2020·Physical Chemistry Chemical Physics : PCCP·Gang HuZuhong Lu
Nov 30, 2006·The Journal of Biological Chemistry·Richard D BennettEmanuel E Strehler
Oct 26, 1999·The Journal of Biological Chemistry·V S ReddyA S Reddy

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