Selective activation of specific PKC isoforms dictating the fate of CD14(+) monocytes towards differentiation or apoptosis

Journal of Cellular Physiology
Yuan-Feng LinYu-Hui Tsai

Abstract

In this study, phorbol-12-myristate-13-acetate (PMA) at low concentrations (<10 nM; L-PMA) induces the differentiation of CD14(+) monocytes into monocyte-derived macrophages (MDMs) while PMA at high concentrations (>100 nM; H-PMA) causes the apoptosis of these cells. The pre-treatment with Go6976 (a PKC-α/β(1) selective inhibitor), not anilinemonoindolylmaleimide [a PKC-β inhibitor (PKC-β inh.)], significantly (P < 0.05) reduces the L-PMA-induced generation of MDMs in the cultured CD14(+) monocytes. On the other hand, either of the above two PKC inhibitors is capable of suppressing the H-PMA-induced apoptosis of CD14(+) monocytes. However, only the inclusion of PKC-β inh., not Go6976, prevents the cells from serum deprivation-induced cell apoptosis. Although the membrane translocation of conventional PKC-α, β(1), and β(2) isoforms was observed in the H-PMA-treated CD14(+) monocytes, only PKC-β(2) exhibits a mitochondrial translocation activity among those PKCs responsive to H-PMA treatment. Moreover, the activation of DEVD-dependent caspases (DEVDase) was also detected in the H-PMA-treated CD14(+) monocytes, indicating the involvement of a caspase-dependent signaling pathway in the H-PMA-induced cell apoptosis of CD14(+) monocy...Continue Reading

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Citations

Oct 19, 2013·Archives of Toxicology·Emanuela CorsiniCorrado L Galli
Apr 24, 2012·Molecular and Cellular Neurosciences·Daorong GuoZheng-Zheng Bao
Nov 7, 2016·Free Radical Biology & Medicine·Francesco GalliNesrin Kartal Özer
Apr 30, 2019·British Journal of Pharmacology·Priscilla DaySamuel J Fountain
Mar 26, 2013·Cellular and Molecular Life Sciences : CMLS·René HuberKorbinian Brand
Aug 19, 2017·Oxidative Medicine and Cellular Longevity·Gabriele PizzinoAlessandra Bitto

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