Selective activation of the mitogen-activated protein kinase subgroups c-Jun NH2 terminal kinase and p38 by IL-1 and TNF in human articular chondrocytes

The Journal of Clinical Investigation
Y GengM Lotz

Abstract

Previous studies suggested that tyrosine kinase activation is an important signal transduction event in the IL-1 response of chondrocytes. The present study identifies the mitogen-activated protein (MAP) kinases extracellular signal-regulated kinase (ERK)-1 and ERK-2 as major tyrosine phosphorylated proteins in IL-1 stimulated chondrocytes. Kinase assays on immunoprecipitates with myelin basic protein as substrate showed that ERK-1 and ERK-2 activation was detectable within 5 min after IL-1 stimulation and decreased to baseline within 60 min. Analysis of other members of the MAP kinase family showed that chondrocytes also express c-Jun NH2 terminal kinase (JNK)-1, JNK-2, and p38 proteins. These kinases were time-dependently activated by IL-1. Among other chondrocyte activators tested, only TNF activated all three of the MAP kinase subgroups. JNK and p38 were not activated by any of the other cytokines and growth factors tested. However, ERK was also activated by PDGF, IGF-1, and IL-6. Phorbol 12-myristate 13-acetate, calcium ionophore, and cAMP analogues only increased ERK activity but had no significant effects on JNK or p38. These results suggest differential activation of MAP kinase subgroups by extracellular stimuli. ERK is...Continue Reading

References

Jun 1, 1992·European Journal of Immunology·E MuñozB T Huber
Jun 1, 1989·Agents and Actions·K I HulkowerC H Evans
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·E R PettipherB Henderson
Nov 15, 1993·Biochemical and Biophysical Research Communications·P G Mitchell, H S Cheung
May 1, 1995·Experimental Cell Research·F J Blanco, M Lotz
Jun 23, 1995·The Journal of Biological Chemistry·M H Cobb, E J Goldsmith
Nov 1, 1994·Trends in Biochemical Sciences·R J Davis
Dec 1, 1994·The Journal of Clinical Investigation·M B GoldringJ F Apperley
May 12, 1994·Nature·J M KyriakisJ R Woodgett

❮ Previous
Next ❯

Citations

Apr 22, 1999·Arthritis and Rheumatism·G S Firestein, A M Manning
Apr 25, 2001·Journal of Cellular Physiology·S KumarJ C Lee
Oct 6, 2009·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Hisashi ShimpoNaoki Ishiguro
Dec 28, 2007·Glycoconjugate Journal·Feng-Ling YangShih-Hsiung Wu
Mar 5, 1999·Immunology Letters·R HanusováA Beschin
Feb 28, 2004·Trends in Cardiovascular Medicine·L New, J Han
May 12, 2000·Alimentary Pharmacology & Therapeutics·K TominagaT Kuroki
Mar 13, 2003·European Journal of Biochemistry·Megumi Funakoshi-TagoTadashi Kasahara
Apr 13, 2000·The European Journal of Neuroscience·G D'ArcangeloF Benfenati
May 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Shuhei OtsukiMartin K Lotz
Aug 18, 2004·Current Opinion in Rheumatology·Francis Berenbaum
Jul 25, 2009·American Journal of Physiology. Cell Physiology·Wei LiMichael B Reid
Nov 1, 2005·Mediators of Inflammation·Riina NieminenEeva Moilanen
Aug 10, 2010·BMC Complementary and Alternative Medicine·Karsten GavénisUlrich Schneider
Dec 20, 2008·Arthritis Research & Therapy·Nadia ZayedHassan Fahmi
Sep 8, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R Tonon, P D'Andrea
Jan 22, 2004·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Charles J Malemud
Feb 28, 2004·American Journal of Pharmacogenomics : Genomics-related Research in Drug Development and Clinical Practice·Mukundan G AtturAshok R Amin
May 30, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tonia VincentJeremy Saklatvala
Feb 15, 2003·Thrombosis Research·Chong-Hua ChenTien-Shang Huang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.