Selective adherence of non-typeable Haemophilus influenzae (NTHi) to mucus or epithelial cells in the chinchilla eustachian tube and middle ear

Microbial Pathogenesis
N Miyamoto, L O Bakaletz

Abstract

Frozen sections of chinchilla Eustachian tube (ET) and middle ear mucosa were incubated with either FITC-labeled non-typeable Haemophilus influenzae (NTHi) or Bordetella pertussis. The number of bacteria adherent to "roof" vs "floor" regions was compared for each of three anatomic portions of the ET and for middle ear epithelium noting whether bacteria adhered to mucus or to epithelial cells. NTHi strains adhered significantly greater to mucus in the ET lumen whereas B. pertussis preferentially adhered to epithelial cells lining the ET (P < or = 0.05). A non-fimbriated isogenic mutant of NTHi adhered significantly less to mucus than the parental isolate at all sites of the ET floor (P < or = 0.05). Isolated fimbrin protein adhered to ET mucus and blocked adherence of whole organisms. Treatment with the mucolytic agent N-acetyl-L-cysteine resulted in significantly reduced adherence of NTHi to mucus (P < or = 0.001) and eliminated the ability to detect binding of isolated fimbrin protein. N-acetyl-L-cysteine treatment did not affect adherence of either B. pertussis or NTHi to epithelial cells. These data indicated that NTHi may mediate ascension of the ET from the nasopharynx primarily via adherence to and growth in mucus overlyi...Continue Reading

Citations

Oct 17, 2012·Infection and Immunity·Forrest K RaffelKevin M Mason
May 19, 2000·Infection and Immunity·M KubietA Smith
Dec 23, 2003·Infection and Immunity·W Edward SwordsJessica VonCannon
Feb 2, 2013·Clinical and Translational Medicine·Paul King
May 26, 2017·Critical Reviews in Microbiology·Krishna Bajee SriramAllan W Cripps
May 1, 2009·Proteomics·Mingguang ZhouMeilin Jin
Jun 20, 2014·Pathogens and Disease·Siva WuPaul Webster
Aug 7, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Laura A Novotny, Lauren O Bakaletz
Jul 3, 2021·Biomolecules·Patrycja BurzyńskaEwa Jaśkiewicz

❮ Previous
Next ❯

Related Concepts