Nov 1, 1994

Selective adhesion of platelets on a polyion complex composed of phospholipid polymers containing sulfonate groups and quarternary ammonium groups

Journal of Biomedical Materials Research
K IshiharaN Nakabayashi


We investigated the effects of electrical charges on cell-polymer interactions of poly[2-methacryloyloxyethyl phosphorylcholine(MPC)-co-n-butyl methacrylate (BMA)] (PMB) having excellent blood compatibility, by copolymerizing anionic or cationic methacrylates with MPC and BMA. A polyion complex (PIC) composed of anionic and cationic MPC copolymers was also prepared. When the cell adhesion on these polymer surfaces from rabbit whole blood was evaluated, we observed a considerable reduction in cell adhesion on the MPC copolymers compared with that on poly(BMA), even when the MPC copolymer was electrically charged. On the other hand, many platelets selectively adhered to the PIC surface from whole blood, but the adherent platelets maintained a discoid shape. The amount of adenosine triphosphate (ATP) in platelets adherent on the PMB or the PIC from a platelet-rich plasma (PRP) was more than 75% of that in the original PRP, which indicated that the activity of these platelets remained high. However, in the platelets adherent to poly(BMA), only a small amount of ATP remained. Protein adsorption on the polymer surface from human plasma was investigated using a gold-colloid-labeled immunoassay against albumin gamma-globulin, and fibri...Continue Reading

  • References19
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Mentioned in this Paper

Platelet Adhesiveness
Pulmonary Fibrosis
Butyl methacrylate
Adenosine Triphosphate, Chromium Ammonium Salt
Scanning Electron Microscopy
2-methacryloyloxyethyl phosphorylcholine

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