PMID: 11330912May 2, 2001Paper

Selective adsorption of biopolymers on zeolites

Chemistry : a European Journal
Masayoshi MatsuiKengo Sakaguchi

Abstract

Zeolites adsorb biopolymers on their surface and may be suitable as a new type of chromatographic carrier material for proteins, nucleic acids, and their conjugates. We report here various parameters that influence the adsorption of biopolymers on synthesized zeolites with regard to the Si/Al2 ratio and three-dimensional structure. There are three physicochemical principles that may underly the adsorption: 1) below the isoelectric point (pI), mainly Coulombic attraction similar to ion-exchange chromatography; 2) at pI, hydrophobic interactions (a kind of van der Waals attraction) plus the three-dimensional mesopore structure; and 3) above pI, the sum of the Coulombic repulsion and attraction forces, such as the hydrophobic interaction, and also substitution reaction of water on the Al molecule with a protein amino-base. At high Si/Al2 ratio in the presence of a small amount of Al and with mesopores between the zeolite particles, maximal adsorption was seen at pI and was suggested to be dependent on the number of hydrophobic interaction points on the mesopores, and their morphology. The application of zeolites to biochemistry and biotechnology is also discussed.

References

Dec 1, 1994·Protein Expression and Purification·D KlintH Eriksson
Jul 1, 1997·Protein Expression and Purification·D Klint, H Eriksson
May 17, 1999·Angewandte Chemie·Yoshikatsu AkiyamaKengo Sakaguchi

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Citations

Jan 20, 2005·Applied Microbiology and Biotechnology·Kengo SakaguchiFujio Mizukami
Oct 12, 2013·Advances in Colloid and Interface Science·Nils CarlssonBjörn Åkerman
Dec 7, 2013·ACS Applied Materials & Interfaces·Xiaohong SunChunming Zheng
Jun 18, 2011·Expert Review of Proteomics·Mingxia GaoXiangmin Zhang
Nov 13, 2013·Dalton Transactions : an International Journal of Inorganic Chemistry·Jinjin ZhaoJianlin Shi
Mar 8, 2017·Nanoscale Research Letters·Berna Ozansoy KasapBurcu Akata Kurc
Jun 28, 2014·Applied Biochemistry and Biotechnology·Andrew CareAnwar Sunna
Apr 9, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Kai GuoEdmond Magner
Feb 4, 2005·Journal of Separation Science·Tibor MackoJoeri F Denayer
Dec 1, 2005·Analytical Biochemistry·Hiroyuki ChikuKengo Sakaguchi
Sep 7, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·John E Krohn, Michael Tsapatsis
Dec 15, 2012·New Biotechnology·Anwar SunnaPeter L Bergquist

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