PMID: 825468Oct 1, 1976

Selective adsorption of heterophile polyglycerophosphate antigen from antigen extracts of Streptococcus mutans and other gram-positive bacteria

Infection and Immunity
S HamadaH D Slade

Abstract

Hot saline extracts of Streptococcus mutans have been shown to contain antigenic substances which occasionally react nonspecifically with some antisera against whole cells of various serological groups and types of streptococci. Chromatography of the extract of S. mutans strain MT703 (serotype e) on a diethylaminoethyl-Sephadex A-25 column gave two principal antigens. One antigen was eluted without adsorption to the resin and was identified as the serotype-specific polysaccharide. The other antigen, which contained a large quantity of phosphorus, was absorbed to and released from the resin by gradient elution. It was reactive against the antisera specific for polyglycerophosphate (PGP) from group A Streptococcus pyogenes and/or S. mutans strain Ingbritt (type c). The PGP antigen was further purified by gel filtration with Sephadex G-75. Two peaks, PGP-1, and PGP-2, were obtained. Each possessed the same antigenic specificity to anti-PGP serum as shown by immunodiffusion. Chemical analyses revealed that the molar ratio of phosphorus to glycerol in both was about 1:1, although the protein content between the two was significantly different. PGP antigen was found to be widely distributed in hot saline extracts from various gram-po...Continue Reading

Citations

Jan 19, 2008·Journal of Medical Microbiology·Wen-Chuan HuangJean-San Chia

Related Concepts

Antigens, Bacterial
Ion-Exchange Chromatography Procedure
Calcium Glycerophosphate
Immunosorbent Techniques
Lactobacillus
Staphylococcus aureus
Streptococcus
Enterococcus faecalis
Lactococcus lactis subsp. lactis
Streptococcus mutans

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