PMID: 2107548Mar 1, 1990Paper

Selective amplification of an mRNA and related pseudogene for a human ADP-ribosylation factor, a guanine nucleotide-dependent protein activator of cholera toxin

Proceedings of the National Academy of Sciences of the United States of America
L MonacoM Vaughan

Abstract

ADP-ribosylation factors (ARFs) are approximately 20-kDa proteins that act as GTP-dependent allosteric activators of cholera toxin. With deoxyinosine-containing degenerate oligonucleotide primers corresponding to conserved GTP-binding domains in ARFs, the polymerase chain reaction (PCR) was used to amplify simultaneously from human DNA portions of three ARF genes that include codons for 102 amino acids, with intervening sequences. Amplification products that differed in size because of differences in intron sizes were separated by agarose gel electrophoresis. One amplified DNA contained no introns and had a sequence different from those of known ARFs. Based on this sequence, selective oligonucleotide probes were prepared and used to isolate clone psi ARF 4, a putative ARF pseudogene, from a human genomic library in lambda phage EMBL3. Reverse transcription-PCR was then used to clone from human poly(A)+ RNA the cDNA corresponding to the expressed homolog of psi ARF 4, referred to as human ARF 4. It appears that psi ARF 4 arose during human evolution by integration of processed ARF 4 mRNA into the genome. Human ARF 4 differs from previously identified mammalian ARFs 1, 2, and 3. Hybridization of ARF 4-specific oligonucleotide pro...Continue Reading

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Citations

Dec 11, 1996·Biochimica Et Biophysica Acta·O SaitohH Nakata
Feb 13, 2001·Plant Science : an International Journal of Experimental Plant Biology·A Kobayashi-UeharaH Handa
Jan 1, 1997·Cellular Signalling·J A Boutin
Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·S C TsaiM Vaughan
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J ClarkR A Kahn
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·J X HongM Vaughan
Dec 31, 1991·Biochemical and Biophysical Research Communications·M MartinezR Alvarez-Gonzalez
May 26, 1998·Australian and New Zealand Journal of Public Health·E van BeurdenE Garner
Jan 10, 1998·The Journal of Biological Chemistry·F J LeeM Vaughan
Dec 4, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yawei LiRichard A Kahn
Nov 17, 2005·DNA Sequence : the Journal of DNA Sequencing and Mapping·Lei WangYony-Jun Wang

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