PMID: 9192651Jun 24, 1997Paper

Selective amplification via biotin- and restriction-mediated enrichment (SABRE), a novel selective amplification procedure for detection of differentially expressed mRNAs

Proceedings of the National Academy of Sciences of the United States of America
D J LaveryU Schibler

Abstract

We present a novel subtractive enrichment protocol for the identification of differentially expressed mRNA species. This procedure, called SABRE (selective amplification via biotin- and restriction-mediated enrichment), uses selective streptavidin-biotin affinity and restriction enzyme site reconstitution to enrich for cDNA species more abundant in one population than in another. Analysis of liver cDNA from a mouse strain expressing the neomycin resistance gene demonstrated that this procedure is capable of identifying species present in one population but absent from another. Furthermore, experiments to identify genes with circadian expression patterns in mouse liver demonstrated that SABRE is capable of detecting even modest 2- to 10-fold differences in accumulation of moderately rare mRNA species, representing as little as 0.03% of total mRNA. These experiments identified the gene encoding coumarin 7-hydroxylase as displaying circadian expression in mouse liver.

References

Jan 1, 1992·Journal of Cell Science. Supplement·J WuarinU Schibler
May 1, 1987·Human Genetics·J R McGillJ W Drysdale
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H Aviv, P Leder
Jan 1, 1974·Methods in Enzymology·R J BrittenB R Neufeld
Oct 20, 1995·Science·V E VelculescuK W Kinzler
Jun 25, 1995·Nucleic Acids Research·R D Miller, R Riblet
Jan 11, 1995·Nucleic Acids Research·J J MilnerP J Dominy
Oct 25, 1994·Nucleic Acids Research·J ZengD Hamer
Oct 1, 1994·Trends in Genetics : TIG·M Gorman, B S Baker
Jan 1, 1993·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·J H Fentem, J R Fry
Jan 1, 1994·Archives of Biochemistry and Biophysics·H OgawaM Fujioka
Jan 1, 1993·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·D Sheehan, J P Casey
Jan 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·L StubbsL Lopez-Molina

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Citations

Mar 29, 2005·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Shouxin LiXiaomei Lei
Mar 17, 2001·Journal of Immunological Methods·C D GreenD A Lewin
Nov 14, 1997·Current Opinion in Biotechnology·M B Soares
Mar 6, 2003·Gene Expression Patterns : GEP·Douglas B Menke, David C Page
Jul 2, 1999·Journal of Sleep Research·U Schibler, M Tafti
Jan 16, 1999·Nucleic Acids Research·K MalhotraP A Schueler
Mar 27, 2001·Nucleic Acids Research·P LichtlenW Schaffner
Jun 8, 1999·The Journal of Clinical Investigation·S KerstenW Wahli
Feb 13, 2001·Biochemical and Biophysical Research Communications·P ViitalaH Raunio
Jan 17, 2002·Proceedings of the National Academy of Sciences of the United States of America·C E MerrillL J Zwiebel
Jun 12, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Michael StockFlorian Otto
Jan 13, 2006·The Journal of Urology·Ricardo Saban, Hsueh-Kung Lin
Mar 15, 2003·Acta Orthopaedica Scandinavica·Gregor Balaburski, J Patrick O'Connor
Mar 30, 2001·Nature Genetics·P J WangD C Page
Jul 15, 2003·Journal of Medical Virology·Larry G BirkenmeyerSuresh M Desai
Jun 20, 2006·Molecular and Cellular Biology·Skirmantas KriaucionisAdrian Bird
Sep 5, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S KerstenW Wahli
Jun 13, 2001·Oncogene·P K Vogt

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