Selective and mild fractionation of microalgal proteins and pigments using aqueous two-phase systems

Journal of Chemical Technology and Biotechnology
Catalina A Suarez RuizC van den Berg

Abstract

Microalgal biomass is generally used to produce a single product instead of valorizing all of the cellular components. The biomass production and downstream processes are too expensive if only one product is valorized. A new approach was proposed for the simultaneous and selective partitioning of pigments and proteins from disrupted Neochloris oleoabundans cultivated under saline and freshwater conditions. An aqueous two-phase system composed of polyethylene glycol and cholinium dihydrogen phosphate selectively separated microalgal pigments from microalgal proteins. 97.3 ± 1.0% of lutein and 51.6 ± 2.3% of chlorophyll were recovered in the polymer-rich phase. Simultaneously, up to 92.2 ± 2.0% of proteins were recovered in a third phase (interface) in between the aqueous phases (interface). The recovered proteins, including Rubisco with a molecular weight of ∼560 kDa, seem to be intact and pigments did not suffer degradation, demonstrating the mildness of this system for fractionating microalgal biomolecules. The ability of aqueous two-phase systems (ATPSs) to simultaneously and efficiently fractionate different biomolecules in a mild manner from disrupted microalgae is demonstrated. This is an important step towards the develop...Continue Reading

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Citations

May 8, 2020·Critical Reviews in Biotechnology·Sambit SarkarKalyan Gayen
Dec 17, 2020·Comprehensive Reviews in Food Science and Food Safety·Leonardo M de Souza MesquitaVeridiana V de Rosso
Apr 3, 2021·Critical Reviews in Food Science and Nutrition·Cassamo Ussemane MussagyAnna Maria Kot
Mar 18, 2021·Trends in Biotechnology·Michel H M EppinkRene H Wijffels

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Methods Mentioned

BETA
Size Exclusion Chromatography
Assay
electrophoresis

Software Mentioned

Statistica
GE Unicorn

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