Selective aptamer-based control of intraneuronal signaling

Angewandte Chemie
Sabine LennarzGünter Mayer

Abstract

Cellular behavior is orchestrated by the complex interactions of a myriad of intracellular signal transduction pathways. To understand and investigate the role of individual components in such signaling networks, the availability of specific inhibitors is of paramount importance. We report the generation and validation of a novel variant of an RNA aptamer that selectively inhibits the mitogen-activated kinase pathway in neurons. We demonstrate that the aptamer retains function under intracellular conditions and that application of the aptamer through the patch-clamp pipette efficiently inhibits mitogen-activated kinase-dependent synaptic plasticity. This approach introduces synthetic aptamers as generic tools, readily applicable to inhibit different components of intraneuronal signaling networks with utmost specificity.

References

Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·H ShiJ T Lis
Jan 27, 2000·Cell·T Hunter
Oct 4, 2000·The Journal of Biological Chemistry·C N Prowse, J Lew
Nov 30, 2000·Chemistry & Biology·S D SeiwertO C Uhlenbeck
Apr 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·G MayerM Famulok
May 1, 2001·Biochemistry·L A Cassiday, L J Maher
Oct 18, 2002·Nucleic Acids Research·J Colin CoxAndrew D Ellington
Dec 10, 2002·Science·G ManningS Sudarsanam
Feb 21, 2004·Nature Reviews. Neuroscience·Gareth M Thomas, Richard L Huganir
Jun 15, 2004·Current Opinion in Neurobiology·J David Sweatt
Jul 27, 2004·Science·Christophe BernardDaniel Johnston
Jul 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Mirko G TheisMichael Famulok
Nov 13, 2008·The Journal of Physiology·J Amiel RosenkranzDaniel Johnston
Jul 25, 2009·Journal of Alzheimer's Disease : JAD·Hans-Wolfgang KlafkiJens Wiltfang
May 18, 2011·Nucleic Acids Research·H Hans SalamancaJohn T Lis
May 10, 2012·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Robert Roskoski
Sep 6, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Margaret J LangeDonald H Burke
Jul 9, 2013·Molecular Neurobiology·Kazuhiro A FujitaRudi Balling

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Citations

Jul 3, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hongguang Sun, Youli Zu
Oct 11, 2016·Chemical Society Reviews·Zijian ZhouXiaoyuan Chen

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