PMID: 8939470Oct 25, 1996Paper

Selective attenuation of mu-opioid receptor-mediated effects in rat sensory neurons by intrathecal administration of antisense oligodeoxynucleotides

Neuroscience Letters
Sachia G KhasarJ D Levine

Abstract

To test the hypothesis that the expression of specific proteins on peripheral terminals of primary afferents can be attenuated by intrathecal administration of antisense oligodeoxynucleotides (ODNs), we administered ODNs antisense to the mu-opioid receptor to male Sprague-Dawley rats via chronically implanted intrathecal cannulae. Antisense but not mismatch ODN treatment significantly decreased peripheral (D-Ala2, N-Me-Phe4, Gly5-ol)-enkephalin (DAMGO) inhibition of prostaglandin E2 (PGE2) hyperalgesia. Antisense treatment affected neither the magnitude of PGE2 hyperalgesia nor the antinociception produced by a peripherally administered adenosine A1-agonist. The antinociceptive effects of DAMGO was fully recovered 8 days after cessation of ODN treatment. DAMGO-induced inhibition of voltage-gated Ca2+ currents (VGCC), in cultured dorsal root ganglion (DRG) neurons from rats treated with ODNs, was also significantly reduced by antisense but not mismatch ODNs. Taken together, these observations suggest that intrathecal administration of antisense ODNs can be used to study the function of proteins present in the peripheral terminals of primary afferent neurons.

Citations

May 10, 2003·Brain Research. Brain Research Reviews·Dirk Van OekelenJosée E Leysen
Dec 1, 1998·Neuroscience Letters·S G KhasarJ D Levine
Apr 24, 2014·The Journal of Pain : Official Journal of the American Pain Society·Pedro Alvarez, Jon D Levine
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·M JiangL Birnbaumer
Jul 8, 1999·Proceedings of the National Academy of Sciences of the United States of America·F PorrecaJ C Hunter
Jul 8, 1999·Proceedings of the National Academy of Sciences of the United States of America·M S Gold
Apr 23, 2015·Pharmacology, Biochemistry, and Behavior·Guilherme R SouzaSergio H Ferreira
Mar 3, 1998·Journal of Molecular Neuroscience : MN·O Ashur-FabianI Gozes
Aug 24, 2010·The European Journal of Neuroscience·Pedro AlvarezPaul G Green
Apr 11, 2021·Proceedings of the National Academy of Sciences of the United States of America·Judith PradoNiels Eijkelkamp
Mar 18, 2008·The Journal of Pain : Official Journal of the American Pain Society·Olayinka A DinaPaul G Green

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antisense Oligonucleotides: ND

This feed focuses on antisense oligonucleotide therapies such as Inotersen, Nusinursen, and Patisiran, in neurodegenerative diseases including amyotrophic lateral sclerosis.