Selective binding and enrichment for low-molecular weight biomarker molecules in human plasma after exposure to nanoporous silica particles

Proteomics
Rosa TerraccianoSalvatore Venuta

Abstract

The present manuscript describes a biomarker capturing strategy based on nanoporous silica particles. The method is shown to enrich the yield of species in the low-molecular weight proteome (LMWP), allowing detection of small peptides in the low-nanomolar range. Plasma samples were exposed to the silica particles, and the captured molecular species were profiled using MALDI-TOF. Mass spectra of the silica-treated human plasma samples showed a significant enrichment in MALDI-TOF protein profiles in the LMWP. Preliminary results indicated good level of reproducibility in plasma profiles with CVs on peak heights ranging from 6.3 to 14.7%. The MALDI-TOF signature changed significantly when the characteristics of the nanoporous silica were altered. The facile sample pretreatment before MS analysis, coupled to the potential for tailoring the surface properties of silica supports, hold promise for improving the recovery of low-abundance serum biomarkers.

References

Jul 16, 1992·Nature·X M He, D C Carter
Jan 1, 1985·Advances in Protein Chemistry·T Peters
Jun 23, 2001·Biosensors & Bioelectronics·T A DesaiM Ferrari
Feb 28, 2002·Lancet·Emanuel F PetricoinLance A Liotta
Mar 8, 2003·Proceedings of the National Academy of Sciences of the United States of America·John B WelshGarret M Hampton
Aug 15, 2003·Molecular & Cellular Proteomics : MCP·Radhakrishna S TirumalaiTimothy D Veenstra
Oct 31, 2003·Nature·Lance A LiottaEmanuel Petricoin
Nov 15, 2003·Journal of Biomedicine & Biotechnology·Guru Reddy, Enrique A. Dalmasso
Feb 6, 2004·Disease Markers·Arpita I MehtaLance A Liotta
Mar 5, 2004·Journal of the National Cancer Institute·Eleftherios P Diamandis
Sep 21, 2004·Biomedical Microdevices·David H GehoLance A Liotta
Jan 25, 2005·Molecular and Cellular Endocrinology·Zhen XiaoHaleem J Issaq
Mar 2, 2005·Nature Reviews. Cancer·Mauro Ferrari
Mar 15, 2005·Analytical Chemistry·E P GoG Siuzdak
Jul 16, 2005·Molecular & Cellular Proteomics : MCP·N Leigh Anderson
Jul 30, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Richard J RobbinsPaul Tempst
Jul 30, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Shahid MianDirk Schadendorf
Aug 9, 2005·Journal of Proteome Research·Josep VillanuevaPaul Tempst
Aug 16, 2005·Clinical Chemistry·Mark S LowenthalLance A Liotta
Oct 11, 2005·Journal of Proteome Research·Anastasia K YocumIan A Blair
Jun 5, 1992·Biotechnology and Bioengineering·B L SteadmanR V Lewis

❮ Previous
Next ❯

Citations

Oct 25, 2013·Analytical and Bioanalytical Chemistry·Liping LiHuwei Liu
Aug 6, 2011·Analytical Chemistry·Michelle Y Chen, Michael J Sailor
Jul 4, 2007·Nature Clinical Practice. Gastroenterology & Hepatology·Shahida DinJack Satsangi
Nov 10, 2006·Nature Reviews. Cancer·Emanuel F PetricoinLance A Liotta
Jul 29, 2010·Chemical Communications : Chem Comm·Qianhao MinHanfa Zou
Jan 23, 2010·AJR. American Journal of Roentgenology·Sonia DuttaDavid A Fishman
Jan 23, 2014·Chemical Communications : Chem Comm·Jie TanJianmin Wu
Aug 25, 2007·Nanomedicine·David H GehoLance A Liotta
Aug 23, 2011·Journal of Proteomics·Jinong LiZivana Tezak
May 21, 2011·Journal of Proteomics·Sandipan RaySanjeeva Srivastava
Jun 30, 2010·Journal of Materials Chemistry·Alessandra LuchiniLance A Liotta
Aug 18, 2009·Expert Review of Proteomics·Lianghai HuHanfa Zou
Oct 22, 2011·Drug Discovery Today·Rocco Savino, Rosa Terracciano
Dec 21, 2010·Gynecologic Oncology·Paul S KimReema Zeineldin
Dec 19, 2015·Proteomics. Clinical Applications·Stijn OonkYuri E M van der Burgt
Sep 11, 2007·The Medical Clinics of North America·Amy Pope-HarmanMauro Ferrari
Sep 11, 2007·Drug Discovery Today·Amit S DhamoonNilofer S Azad
Dec 15, 2006·Journal of Chromatography. a·Jose L Luque-Garcia, Thomas A Neubert
Aug 1, 2007·Proteomics. Clinical Applications·Richard R DrakeO John Semmes
Apr 4, 2007·Mass Spectrometry Reviews·Xuewu ZhangFeng Chen
Mar 17, 2010·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Ye HuMauro Ferrari
Mar 12, 2011·Journal of Mass Spectrometry : JMS·Sebastien GallienBruno Domon
Jul 21, 2010·Chemistry : a European Journal·Francesca CasadonteRosa Terracciano
Dec 17, 2014·Proteomics. Clinical Applications·Rosa TerraccianoRocco Savino
Dec 3, 2009·Proteomics·Jungbae KimRichard D Smith
Dec 18, 2014·Integrative Biology : Quantitative Biosciences From Nano to Macro·Giovanni MarinaroFrancesco Gentile
May 3, 2016·Clinica Chimica Acta; International Journal of Clinical Chemistry·Joanna HajdukZenon J Kokot
Sep 22, 2006·Biomaterials·A Jasper NijdamMauro Ferrari

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.