PMID: 11929231Apr 4, 2002Paper

Selective binding of mannose-encapsulated gold nanoparticles to type 1 pili in Escherichia coli

Journal of the American Chemical Society
Chun-Cheng LinYi-Chun Wu

Abstract

The synthesis, characterization and biological application of mannose encapsulated gold nanoparticles (m-AuNP) are reported. m-AuNP is well dispersed and very stable without aggregation in the media of broad ion strength and pH ranges. The selective binding of m-AuNP to the mannose adhesin FimH of bacterial type 1 pili is demonstrated using transmission electron microscopy. The competition assay with free mannose suggests that m-AuNP binds FimH better than free mannose does. This work demonstrates that carbohydrate attached nanoparticles can be used as an efficient affinity label and a multi-ligand carrier in a biological system.

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