Selective capture and sensitive fluorometric determination of Pseudomonas aeruginosa by using aptamer modified magnetic nanoparticles

Mikrochimica Acta
Zitao ZhongLi Jia

Abstract

A fluorometric assay is described for the detection of the food pathogen Pseudomonas aeruginosa (P. aeruginosa). It is based on the hybridization of aptamer and fluorescein-labeled complementary DNA (FAM-cDNA) in combination with magnetic separation. In the absence of P. aeruginosa, FAM-cDNA is assembled on the surface of aptamer modified magnetic particles (MNPs) via hybridization between aptamer and cDNA. Upon addition of P. aeruginosa, FAM-cDNA is replaced by the bacteria and released from the MNPs since the aptamer preferentially binds to bacteria. After magnetic separation, the amount of bacteria can be quantified by determination of the fluorescence intensity (λexc/em = 494/525 nm) of the supernatant containing the released FAM-cDNA. This kind of assay allows for both selective enrichment and sensitive fluorometric determination of bacteria in a single step. The assay has a response to the logarithm of P. aeruginosa concentration that is linear in the range between 10 and 108 cfu·mL-1, with a detection limit as low as 1 cfu·mL-1. The detection process can be finished within <1.5 h. The feasibility of the assay was verified by detecting P. aeruginosa in spiked food samples. Graphical abstract Hybridization of aptamer and c...Continue Reading

References

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Citations

Jan 7, 2020·Annual Review of Food Science and Technology·Lei Mei, Qin Wang
Jul 28, 2020·International Journal of Molecular Sciences·Nevina E Trunzo, Ka Lok Hong
Feb 9, 2021·World Journal of Microbiology & Biotechnology·Danliang LiLili Chen
Jan 9, 2021·Frontiers in Microbiology·Xin ZhengXiaobo Hu
Mar 9, 2021·Frontiers in Microbiology·Mariam Saad, Sebastien P Faucher
Jul 16, 2021·Food Science & Nutrition·Mingcan QinShujun Wang

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