Selective cholinergic depletion in medial septum leads to impaired long term potentiation and glutamatergic synaptic currents in the hippocampus.

PloS One
Patrick M KanjuVishnu Suppiramaniam

Abstract

Cholinergic depletion in the medial septum (MS) is associated with impaired hippocampal-dependent learning and memory. Here we investigated whether long term potentiation (LTP) and synaptic currents, mediated by alpha-amino-3-hydroxy-5-methyl-isoxazole-4-propionate (AMPA) and N-methyl-D-aspartate (NMDA) receptors in the CA1 hippocampal region, are affected following cholinergic lesions of the MS. Stereotaxic intra-medioseptal infusions of a selective immunotoxin, 192-saporin, against cholinergic neurons or sterile saline were made in adult rats. Four days after infusions, hippocampal slices were made and LTP, whole cell, and single channel (AMPA or NMDA receptor) currents were recorded. Results demonstrated impairment in the induction and expression of LTP in lesioned rats. Lesioned rats also showed decreases in synaptic currents from CA1 pyramidal cells and synaptosomal single channels of AMPA and NMDA receptors. Our results suggest that MS cholinergic afferents modulate LTP and glutamatergic currents in the CA1 region of the hippocampus, providing a potential synaptic mechanism for the learning and memory deficits observed in the rodent model of selective MS cholinergic lesioning.

References

May 1, 1992·The Journal of Physiology·T A Pitler, B E Alger
Aug 10, 1992·Behavioural Brain Research·M M'Harzi, L E Jarrard
May 1, 1990·Trends in Neurosciences·M Stewart, S E Fox
Dec 1, 1990·Behavioral Neuroscience·B S Givens, D S Olton
Apr 1, 1988·Behavioral Neuroscience·J E Kelsey, B A Landry
Jul 30, 1982·Science·R T BartusA S Lippa
Apr 1, 1995·Current Opinion in Neurobiology·T G Aigner
Oct 1, 1994·Journal of Neurophysiology·J M Auerbach, M Segal
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J Ambros-Ingerson, G Lynch
Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S MarenR F Thompson
Nov 1, 1995·Brain Research. Brain Research Reviews·A Blokland
Apr 15, 1996·The Journal of Physiology·J M Auerbach, M Segal
Jan 1, 1997·Annual Review of Psychology·B J Everitt, T W Robbins
Mar 7, 1998·Journal of Neuroscience Methods·M W JohnsonJ B Watson
May 30, 1998·Journal of Neuroscience Methods·T SaitoT Nemoto
Jul 9, 1998·Nature·T A BenkeG L Collingridge
Oct 13, 1998·Pharmacology, Biochemistry, and Behavior·E D LevinN C Christopher
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·V DerkachT R Soderling
Jul 22, 1999·Annals of the New York Academy of Sciences·K A RadcliffeJ A Dani
Aug 26, 1999·Trends in Cognitive Sciences·M E Hasselmo
Apr 21, 2001·Psychopharmacology·A CiameiC Castellano
Sep 6, 2001·Journal of Neurophysiology·S L Morgan, T J Teyler

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Citations

Oct 10, 2012·Proceedings of the National Academy of Sciences of the United States of America·Amanda C MartynVania F Prado
May 2, 2021·Brain Research Bulletin·Mu-Hsuan ChenJeng-Rung Chen

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Methods Mentioned

BETA
dissection

Software Mentioned

Mini Analysis
pClamp
LTP
pClamp9

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