Selective counting and sizing of single virus particles using fluorescent aptamer-based nanoparticle tracking analysis

Nanoscale
Zoltán SzakácsRóbert E Gyurcsányi

Abstract

Detection and counting of single virus particles in liquid samples are largely limited to narrow size distribution of viruses and purified formulations. To address these limitations, here we propose a calibration-free method that enables concurrently the selective recognition, counting and sizing of virus particles as demonstrated through the detection of human respiratory syncytial virus (RSV), an enveloped virus with a broad size distribution, in throat swab samples. RSV viruses were selectively labeled through their attachment glycoproteins (G) with fluorescent aptamers, which further enabled their identification, sizing and counting at the single particle level by fluorescent nanoparticle tracking analysis. The proposed approach seems to be generally applicable to virus detection and quantification. Moreover, it could be successfully applied to detect single RSV particles in swab samples of diagnostic relevance. Since the selective recognition is associated with the sizing of each detected particle, this method enables to discriminate viral elements linked to the virus as well as various virus forms and associations.

References

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Citations

Nov 26, 2019·Human Gene Therapy Methods·Manuela GastBoris Mizaikoff
Sep 5, 2020·Biological Procedures Online·Elena EkramiMatin Mamoudifard
Jul 25, 2019·Frontiers in Microbiology·Xinran ZouLingxiang Mao
Jan 23, 2021·Methods and Applications in Fluorescence·Swarupa ChatterjeeChristian Blum
Dec 29, 2020·Proceedings. Mathematical, Physical, and Engineering Sciences·José Alvim BerkenbrockSven Achenbach
Oct 9, 2021·Advanced Materials·Zhaowei ZhangUtkan Demirci
Jan 27, 2022·Nanoscale·Zsófia BognárRóbert E Gyurcsányi

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Methods Mentioned

BETA
genotyping
flow cytometry
AFM
light scattering

Software Mentioned

NanoSight NTA
NTA

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