Selective cultivation of normal human sebocytes in vitro; a simple modified technique for a better cell yield

Experimental Dermatology
M Badawy Abdel-Naser

Abstract

Selective cultivation of normal human sebocytes is essential for better understanding of drug pharmacokinetics and diseases of the pilosebaceous apparatus. In the present study, sebocytes are selectively cultivated in vitro using modified MCDB 153 medium to which cholera toxin (1 x 10(-9) M), crude bovine pituitary extract (70 micro g/ml), epidermal growth factor (10 ng/ml), basic fibroblast growth factor (2 ng/ml), hydrocortisone (1.4 x 10(-6) M), insulin (10 micro g/ml), fetal bovine serum (10%), and antibiotics were added. To maintain contact of the floating sebaceous lobules with culture plate, two methods have been adopted. (i) Using little amount of culture medium that barely covers the gland lobules with frequent dropping of the medium to replace the loss by evaporation (almost every 2 h). (ii) Placing a sterile glass slide cover over the gland lobules in the presence of enough culture medium. Both methods are performed for the first 72 h of inoculation, when cells are seen outgrowing from sebaceous lobules. Both populations show the characteristic morphology of sebocytes in culture, namely polygonal shape with abundant cytoplasm resembling basal keratinocytes. As the culture grows older, a vacuolated refractile cytoplas...Continue Reading

References

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Citations

Jan 25, 2013·BMC Dermatology·Adrian J McNairnGéraldine Guasch
Apr 2, 2015·Experimental Dermatology·Georgios NikolakisChristos C Zouboulis
Apr 19, 2012·Experimental Dermatology·Mohamed Badawy Abdel-NaserChristos C Zouboulis
Nov 8, 2018·Cell Proliferation·Anne-France de BengyColin McGuckin

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