Abstract
The system N glutamine (Gln) transporter SN1(SNAT3) is overexpressed in human malignant glioma cells in situ as compared to the adjacent brain tissue or metastases from different organs [Sidoryk, M., Matyja, E., Dybel, A., Zielińska, M., Bogucki, J., Jaskólski, D.J., Liberski, P.P., Kowalczyk, P., Albrecht, J., 2004]. Increased expression of a glutamine transporter SNAT3 is a marker of malignant gliomas. NeuroReport 15, 575-578], but its role in tumor growth as compared to the other Gln transporters is unknown. One of the profound, growth-promoting effects of glial tumor in situ is acidification of the extracellular space. In the kidney SN1(SNAT3) mRNA participates in the adaptation to acidosis. In this study therefore, expression of mRNAs coding for SN1(SNAT3) and other Gln transporters was measured in human (T98G) and rat (C6) glioma cells incubated for 4h in an acidic medium (AI) (pH 6.5). MTT assay revealed no cell loss in AI cells, and intracellular pH (pHi) as measured by a fluorescent probe (BCECF-AM) was slightly alkaline in C6 and T98G cells, indicating that the cells have adapted to AI. AI significantly decreased the SN1(SNAT3) mRNA expression in C6 (a 60% decrease) and T98G cells (a 50% decrease). The decrease retrea...Continue Reading
References
Jul 6, 1992·Molecular and Cellular Biochemistry·M A MedinaI Núñez de Castro
Sep 1, 1991·British Journal of Cancer·J R Griffiths
Feb 1, 1990·Radiology·B HubeschM W Weiner
Dec 16, 1983·Journal of Immunological Methods·T Mosmann
Dec 1, 1993·Annals of Surgery·W W Souba
Aug 2, 1993·FEBS Letters·J C PortaisM Merle
Aug 1, 1996·Annals of Surgery·M WasaW W Souba
Sep 23, 1997·Glia·U SonnewaldA Schousboe
Oct 28, 1998·Physiological Reviews·M PalacínA Zorzano
Jan 5, 2000·Cell·F A ChaudhryR H Edwards
Feb 7, 2001·American Journal of Physiology. Cell Physiology·L A McLeanP M Cala
May 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·W RzeskiC Ikonomidou
Sep 5, 2001·Nature Medicine·T TakanoM Nedergaard
Sep 11, 2001·Journal of Neuroscience Research·A K Bouzier-SoreM Merle
Jan 18, 2002·Journal of Neurochemistry·Kristine GlundeDieter Leibfritz
Feb 19, 2002·The Journal of Physiology·Angelika BröerStefan Bröer
May 9, 2002·American Journal of Physiology. Cell Physiology·Masafumi WasaAkira Okada
Jun 13, 2002·The European Journal of Neuroscience·Jean-Luc BoullandFarrukh A Chaudhry
Oct 10, 2002·American Journal of Physiology. Renal Physiology·Anne M KarinchWiley W Souba
May 14, 2003·Neurochemistry International·Monika DolińskaJan Albrecht
Jul 8, 2003·Pflügers Archiv : European journal of physiology·Bryan Mackenzie, Jeffrey D Erickson
Oct 3, 2003·Trends in Neurosciences·Harald Sontheimer
Apr 20, 2004·Neuroreport·Marta SidorykJan Albrecht
Sep 25, 2004·Glia·Beatriz CubelosFrancisco Zafra
Feb 18, 2005·Journal of the American Society of Nephrology : JASN·Tom Tallak SolbuFarrukh A Chaudhry
Mar 23, 2005·Brain Pathology·Lars TatenhorstWerner Paulus
Citations
Jun 2, 2011·Journal of Pharmaceutical Sciences·Takeo Nakanishi, Ikumi Tamai
Jul 8, 2010·The Journal of Surgical Research·Wen-long YuGuanzhen Yu
May 22, 2009·Journal of Neurochemistry·Marta Sidoryk-WegrzynowiczMichael Aschner
Jul 24, 2012·Glia·El Hassan HamdaniFarrukh Abbas Chaudhry
Dec 7, 2007·Neurochemistry International·Marta ObaraJan Albrecht
Mar 9, 2017·Glia·Alison C ToddBrian Billups
Feb 6, 2020·Cancers·Marta Obara-Michlewska, Monika Szeliga