Selective degradation of reverse gyrase and DNA fragmentation induced by alkylating agent in the archaeon Sulfolobus solfataricus.

Nucleic Acids Research
Anna ValentiMaria Ciaramella

Abstract

Reverse gyrase is a peculiar DNA topoisomerase, specific of hyperthermophilic Archaea and Bacteria, which has the unique ability of introducing positive supercoiling into DNA molecules. Although the function of the enzyme has not been established directly, it has been suggested to be involved in DNA protection and repair. We show here that the enzyme is degraded after treatment of Sulfolobus solfataricus cells with the alkylating agent MMS. MMS-induced reverse gyrase degradation is highly specific, since (i) neither hydroxyurea (HU) nor puromycin have a similar effect, and (ii) topoisomerase VI and two chromatin components are not degraded. Reverse gyrase degradation does not depend on protein synthesis. Experiments in vitro show that direct exposure of cell extracts to MMS does not induce reverse gyrase degradation; instead, extracts from MMS-treated cells contain some factor(s) able to degrade the enzyme in extracts from control cells. In vitro, degradation is blocked by incubation with divalent metal chelators, suggesting that reverse gyrase is selectively degraded by a metal-dependent protease in MMS-treated cells. In addition, we find a striking concurrence of extensive genomic DNA degradation and reverse gyrase loss in MM...Continue Reading

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Citations

Feb 15, 2011·Extremophiles : Life Under Extreme Conditions·Jie LiHua Xiang
Sep 21, 2010·The Journal of Biological Chemistry·Anna ValentiMaria Ciaramella
Dec 15, 2011·The Journal of Biological Chemistry·Giuseppe PeruginoMaria Ciaramella
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Aug 12, 2008·Applied and Environmental Microbiology·José Luis FernándezGermán Bou
Aug 12, 2014·Extremophiles : Life Under Extreme Conditions·Antonella VettoneMaria Ciaramella
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Jan 27, 2011·Biochemical Society Transactions·Anna ValentiMaria Ciaramella
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Jun 13, 2016·Extremophiles : Life Under Extreme Conditions·Xueguo SongYulong Shen
Oct 30, 2016·Biochimica Et Biophysica Acta. General Subjects·Castrese MorroneMaria Ciaramella
Dec 6, 2017·International Journal of Molecular Sciences·Riccardo MiggianoMaria Ciaramella
Jun 24, 2017·International Journal of Molecular Sciences·Wenyuan HanQunxin She
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Jan 16, 2009·Biochemical Society Transactions·Giuseppe PeruginoMaria Ciaramella
Apr 25, 2020·International Journal of Molecular Sciences·Rosanna MattossovichGiuseppe Perugino
Jan 11, 2020·Scientific Reports·Marcello GermoglioAdele Adamo
Jun 28, 2020·Journal of Molecular Biology·Frederic CollinDagmar Klostermeier
Jun 12, 2021·Frontiers in Microbiology·Florence GarnierMarc Nadal

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