Selective depletion of chromosomes in a stable mouse-Chinese hamster hybrid cell line using antisera directed against species-specific cell surface antigens

Journal of Cellular Physiology
J J CollinsI A Macpherson

Abstract

A chromosomally-stable mouse-Chinese hamster hybrid cell line was subjected to five rounds of selection with cytotoxic antisera raised in rabbits against either the parental mouse 3T3 cells or the parental Chinese hamster Wg-1 cells. Routine karyological analysis of clones isolated at each stage of serum selection revealed that treatment with either serum resulted in a limited loss of chromosomes (compared to the untreated hybrid cell cultured in parallel) and that the pattern of chromosome loss could not be correlated with the particular antiserum used for selection. However, more detailed analysis with the SSC-formamide C-banding technique, which identifies chromosomes containing a mouse centromere region, demonstrated that while large-scale chromosome loss was not achieved as a result of antiserum selection, the limited loss of chromosomes did, in fact, reflect a specific depletion of chromosomes in response to treatment with cytotoxic antiserum. Specific chromosomal elimination was shown to occur as early asthe first round of antiserum treatment. Antigenic analysis of the serum-selected clones revealed a quantitative decrease in the expression of the species-specific surface antigens selected against, but no qualitative los...Continue Reading

References

Apr 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·M C WeissL J Scaletta
May 1, 1970·Virology·P Häyry, V Defendi
Dec 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·J Jami, S Grandchamp
Dec 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·T T PuckF T Kao
Sep 15, 1972·International Journal of Cancer. Journal International Du Cancer·C N Wiblin, I A MacPherson
Sep 1, 1973·Journal of the National Cancer Institute·G BarskiB Leon
Dec 15, 1973·Journal of Molecular Biology·H G CoonI B Dawid
Jul 15, 1973·International Journal of Cancer. Journal International Du Cancer·C N Wiblin, I Macpherson
Jul 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·A F GazdarJ D Minna
Jun 1, 1973·Journal of the National Cancer Institute·J W SmithW E Rawls
Mar 16, 1973·Nature·F H Ruddle
Apr 1, 1972·The Journal of Experimental Medicine·G KleinH Harris
Jan 1, 1972·Experimental Cell Research·G Marin, L Pugliatti-Crippa
Sep 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·M C Weiss, H Green
Mar 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·M Cikes, S Friberg
Nov 3, 1971·Nature: New Biology·A WesterveldD Bootsma
Feb 15, 1963·Proceedings of the National Academy of Sciences of the United States of America·M VOGT, R DULBECCO

❮ Previous
Next ❯

Citations

Dec 10, 1982·Annals of the New York Academy of Sciences·D D Pravtcheva, F H Ruddle
Jan 1, 1977·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·J M BoyleP J Smith

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.