PMID: 7018684May 1, 1981Paper

Selective depolymerisation of heparin to produce radio-labelled substrates for sulfamidase, 2-acetamido-2-deoxy-alpha-D-glucosidase, acetyl-CoA:2-amino-2-deoxy-alpha-D-glucoside N-acetyltransferase, and 2-acetamido-2-deoxy-D-glucose 6-sulfate sulfatase

Carbohydrate Research
J J Hopwood, H Elliott

Abstract

Heparin was carboxyl-reduced with NaBT4, and degraded under conditions of acid hydrolysis that selectively cleaved the 2-0-sulfo-L-idopyranosidic linkages. The resulting, radiolabelled-disaccharides and -tetrasaccharides were isolated by gel chromatography, and then fractionated by ion-exchange chromatography, paper chromatography, and paper electrophoresis. Of the nine disaccharides isolated and identified, eight were probably derived from the major repeating-disaccharide unit in heparin (2-deoxy-2-sulfoamino-D-glucosyl 6-sulfate leads to L-idosyluronic acid 2-sulfate). Sodium borotritide reduction and/or HNO2 deamination of these eight disaccharide fractions indicated four to contain L-idopyranose residues and the other four to contain 1,6-anhydro-L-idopyranose residues as terminal units. The latter, terminal unit probably represents a minor component formed during the acid hydrolysis. On the basis of N-acetylation, N-sulfation, and HNO2-deamination studies, and the known positions and configurations of the glycosidic and sulfate linkages in heparin, four disaccharides were identified as 0-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-(1 leads to 4)-L-[6-3H]idopyranose, 0-(2-amino-2-deoxy-alpha-D-glycopyranosyl)-(1 leads to 4)-L-[...Continue Reading

References

Sep 7, 1976·Biochemistry·J E Shively, H E Conrad
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·U KleinK von Figura
Sep 1, 1979·Clinical Science·J J Hopwood, V Muller
Nov 1, 1978·Journal of Clinical Pathology·Y Ben-Yoseph, H L Nadler
May 17, 1977·Biochemistry·J A Cifonelli, J A King
Apr 1, 1979·The Biochemical Journal·I JacobssonK von Figura
Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R D RosenbergL Lam
Jan 1, 1978·Annual Review of Biochemistry·U Lindahl, M Höök
Apr 1, 1977·Clinica Chimica Acta; International Journal of Clinical Chemistry·P Whiteman, E Young
Jun 15, 1977·Clinica Chimica Acta; International Journal of Clinical Chemistry·P Di NataleE F Neufeld
Jun 1, 1977·The Australian Journal of Experimental Biology and Medical Science·W F Carey, A C Pollard
Feb 28, 1978·Biochemical and Biophysical Research Communications·A L Horwitz, A Dorfman
Nov 1, 1978·Clinica Chimica Acta; International Journal of Clinical Chemistry·J N Thompson
Feb 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·A Dorfman, R Matalon
Oct 1, 1974·Carbohydrate Research·T W LimE F Neufeld
Nov 15, 1974·Nature·J F TallmanB Padeh
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·H KresseW Fuchs
Oct 1, 1962·Analytical Biochemistry·T BITTER, H M MUIR
Jan 1, 1952·The Biochemical Journal·W E TREVELYAN, J S HARRISON

❮ Previous
Next ❯

Citations

Oct 1, 1985·European Journal of Biochemistry·P R ClementsJ J Hopwood
Mar 26, 1982·Clinica Chimica Acta; International Journal of Clinical Chemistry·J J Hopwood, H Elliott
Aug 18, 1982·Clinica Chimica Acta; International Journal of Clinical Chemistry·J J Hopwood, H Elliott

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.