Selective detection and phylogenetic diversity of Acaryochloris spp. that exist in association with didemnid ascidians and sponge.

Microbes and Environments
Satoshi Ohkubo, Hideaki Miyashita

Abstract

Acaryochloris spp. are unique cyanobacteria which contain chlorophyll d as the predominant pigment. The phylogenetic diversity of Acaryochloris spp. associated with 7 Prochloron- or Synechocystis-containing didemnid ascidians and 1 Synechococcus-containing sponge obtained from the coast of the Republic of Palau was analyzed; we established a PCR primer set designed to selectively amplify the partial 16S rRNA gene of Acaryochloris spp. even in DNA samples containing a large amount of other cyanobacterial and algal DNAs. Polymerase chain reaction-denaturing gradient gel electrophoresis with this primer set enabled detection of the phyogenetic diversity of Acaryochloris spp. All the ascidian and sponge samples contained Acaryochloris spp. Fourteen phylotypes that were highly homologous (98-100%) with A. marina MBIC11017 were detected, while only 2 phylotypes were detected with our previously developed method for detecting cyanobacteria. The results also revealed that many uncultured phylotypes of Acaryochloris spp. were associated with those didemnid ascidians, since a clonal culture of only 1 phylotype has been established thus far. No specific relationship was found among the Acaryochloris phylotypes and the genera of the ascidi...Continue Reading

References

Aug 1, 1997·Applied and Environmental Microbiology·U NübelG Muyzer
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·Q HuS Itoh
Feb 27, 2004·Nucleic Acids Research·Wolfgang LudwigKarl-Heinz Schleifer
Mar 16, 2004·Science·Akio MurakamiMamoru Mimuro
Jan 8, 2005·Proceedings of the National Academy of Sciences of the United States of America·Scott R MillerA Michelle Wood
Feb 25, 2005·Nature·Michael KühlAnthony W D Larkum
Oct 10, 2006·Applied and Environmental Microbiology·Satoshi OhkuboMamoru Mimuro
Nov 7, 2006·Nature Chemical Biology·Mohamed S DoniaEric W Schmidt
Feb 1, 2007·Antonie van Leeuwenhoek·Ana E Pérez-MatosNadathur S Govind
Apr 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·Tatsuya TomoMamoru Mimuro
May 1, 2007·FEMS Microbiology Ecology·Yi-Wah ChanMartha R J Clokie
Aug 23, 2011·Applied and Environmental Microbiology·Naomi F Montalvo, Russell T Hill

❮ Previous
Next ❯

Citations

Apr 26, 2013·Photosynthesis Research·Patrick LoughlinMin Chen
Nov 13, 2014·Biofouling·Thirumahal MuthukrishnanAlistair A Finnie
Mar 19, 2014·Applied and Environmental Microbiology·Lars BehrendtMichael Kühl
Jun 18, 2017·The ISME Journal·Satoshi Ohkubo, Hideaki Miyashita
May 21, 2017·Biological Research·Syed Lal BadshahSalim S Al-Showiman

❮ Previous
Next ❯

Datasets Mentioned

BETA
AB479412
AB479463

Methods Mentioned

BETA
electrophoresis
PCR
PCRs

Software Mentioned

DNAPARS
BLAST
DNAML
DNADIST
PHYLIP
SEQBOOT
NEIGHBOR
ARB

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.