Selective detection of proteins in mixtures using electrospray ionization mass spectrometry: influence of instrumental settings and implications for proteomics

Analytical Chemistry
Seetharaman VaidyanathanRoyston Goodacre

Abstract

We studied the effects of electrospray mass spectrometric instrumental settings on the relative and absolute detection of individual proteins in a five-component mixture. Conditions that were effective for a given protein could be very poor for the others, and vice versa, such that to a good approximation it was possible to find conditions for selective detection of individual proteins in a complex mixture without prior analytical separation. Some of these could be rationalized on the basis of the known biophysical properties of the individual proteins. The ability to vary the conditions of a mass spectrometric detection method on-line provides an important degree of freedom for the selective detection, and hence discrimination, of individual proteins and peptides in complex mixtures and has implications in proteomics, in particular with respect to top-down strategies for proteomic characterizations.

Citations

Jan 8, 2008·Chemical & Pharmaceutical Bulletin·Gedela SrinubabuMedicherla Narasimha Rao
Jul 3, 2007·Journal of the American Society for Mass Spectrometry·Peter W MurphyDavid M Byers
May 13, 2014·IEEE Transactions on Cybernetics·Kaustuv NagNikhil R Pal
Jun 15, 2005·Nature Reviews. Microbiology·Douglas B KellStephen G Oliver
Oct 26, 2007·Lab on a Chip·Sander Koster, Elisabeth Verpoorte
May 16, 2008·Journal of Separation Science·Guillaume L ErnyAlejandro Cifuentes
Dec 22, 2019·International Journal of Molecular Sciences·Kathiresan PurushothamanIgor Babiak
May 31, 2011·Analytical Chemistry·Elena N KitovaJohn S Klassen
Sep 20, 2011·Analytical Chemistry·Bin HuZhong-Ping Yao

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