Selective diffusion barriers separate membrane compartments.

Biophysical Journal
Andreas BruckbauerDavid Klenerman

Abstract

We have investigated exchange of molecules between different membrane domains on a highly compartmentalized cell, the spermatozoon. Using Alexa Fluor 555-cholera toxin B-subunit we have observed clustering of preexisting GM1 gangliosides which diffused across the anterior acrosome-equatorial segment interface but did not access the postacrosome. By contrast, single lipid and protein molecules readily exchanged between all three domains, although they diffused more slowly on nearing and crossing to the postacrosome. Thus, two types of diffusion interfaces are present on sperm heads, an "open" interface and a "mass filter" interface. The latter seems to be due to a protein-cytoskeleton network.

References

Dec 6, 2006·Proceedings of the National Academy of Sciences of the United States of America·Björn F LillemeierMark M Davis
Jul 16, 2008·Proceedings of the National Academy of Sciences of the United States of America·Daniel LingwoodKai Simons

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Citations

Dec 26, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·David KlenermanSimon J Davis
Dec 26, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Alex RobsonMark C Leake
Dec 4, 2012·International Journal of Molecular Sciences·Marisa L Martin-Fernandez, David T Clarke
May 5, 2011·Asian Journal of Andrology·Pablo E ViscontiAlberto Darszon
Jul 4, 2012·Nature Materials·Jing LiuBo Huang
Aug 15, 2018·Frontiers in Cell and Developmental Biology·Lis C Puga MolinaMariano G Buffone

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