Selective dissociation of non-covalent bonds in biological molecules by laser spray

Journal of Mass Spectrometry : JMS
Atsushi TakamizawaKenzo Hiraoka

Abstract

The laser spray developed in our laboratory was applied to the analysis of bovine serum albumin (BSA), double-stranded DNA (dsDNA) and a protein-DNA complex. The tip of a stainless-steel capillary was irradiated with a 10.6 micro m infrared laser by increasing the laser power from 0 W (electrospray) to 1.4 W. The laser beam was focused to about 0.3 mm at the tip of the stainless-steel capillary. When BSA aqueous solution was irradiated by the laser, highly charged monomer ions were newly observed in addition to the multiply charged ions of non-denatured monomer, dimer and trimer moieties. This indicates that BSA suffers from denaturation on irradiation with an infrared laser in solution. A 1.4 W laser power is not sufficient to cause the complete denaturation of BSA under the present experimental conditions. Whereas dsDNA was found to dissociate almost completely to single-stranded DNA constituents on laser irradiation with a power of 1.2 W, no fragmentation of DNA molecules was observed. For a protein-DNA complex, i.e. a complex of c-Myb DNA binding domain and dsDNA, dissociation of the complex to the component moieties was observed. These findings indicate that the laser spray can selectively dissociate non-covalent complexes...Continue Reading

Citations

Jan 18, 2005·Journal of the American Society for Mass Spectrometry·Satoko AkashiYoshifumi Nishimura
May 24, 2005·Journal of the American Society for Mass Spectrometry·Atsushi TakamizawaKenzo Hiraoka
Feb 24, 2006·Journal of the American Society for Mass Spectrometry·Xiangguo ShiKenzo Hiraoka
Jun 16, 2005·Photomedicine and Laser Surgery
Sep 8, 2005·Photomedicine and Laser Surgery
Oct 4, 2006·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Satoko Akashi
Apr 9, 2008·Rapid Communications in Mass Spectrometry : RCM·Xiangguo ShiSatoko Akashi
Apr 28, 2007·Rapid Communications in Mass Spectrometry : RCM·Masana NakamuraSatoko Akashi
Jul 11, 2006·Journal of Mass Spectrometry : JMS·Xiangguo ShiSatoko Akashi
Jul 18, 2008·Rapid Communications in Mass Spectrometry : RCM·Atsushi TakamizawaKenzo Hiraoka
Feb 24, 2012·Journal of Mass Spectrometry : JMS·Atsushi TakamizawaKenzo Hiraoka
Nov 9, 2004·Journal of Mass Spectrometry : JMS

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