Selective Elimination of Membrane-Damaged Chloroplasts via Microautophagy

Plant Physiology
Sakuya NakamuraMasanori Izumi

Abstract

Plant chloroplasts constantly accumulate damage caused by visible wavelengths of light during photosynthesis. Our previous study revealed that entire photodamaged chloroplasts are subjected to vacuolar digestion through an autophagy process termed chlorophagy; however, how this process is induced and executed remained poorly understood. In this study, we monitored intracellular induction of chlorophagy in Arabidopsis (Arabidopsis thaliana) leaves and found that mesophyll cells damaged by high visible light displayed abnormal chloroplasts with a swollen shape and 2.5 times the volume of normal chloroplasts. In wild-type plants, the activation of chlorophagy decreased the number of swollen chloroplasts. In the autophagy-deficient autophagy mutants, the swollen chloroplasts persisted, and dysfunctional chloroplasts that had lost chlorophyll fluorescence accumulated in the cytoplasm. Chloroplast swelling and subsequent induction of chlorophagy were suppressed by the application of exogenous mannitol to increase the osmotic pressure outside chloroplasts or by overexpression of VESICLE INDUCING PROTEIN IN PLASTID1, which maintains chloroplast envelope integrity. Microscopic observations of autophagy-related membranes showed that swol...Continue Reading

Citations

Nov 16, 2019·Cells·Qinwu ChenCéline Masclaux-Daubresse
Jan 8, 2020·Cells·Omar López-VidalAna Jiménez
Apr 9, 2020·Cells·Katarzyna SieńkoShino Goto-Yamada
Apr 24, 2020·Journal of Integrative Plant Biology·Hua QiShi Xiao
Dec 7, 2018·Open Biology·Jie Tang, Diane C Bassham
Jan 9, 2019·Frontiers in Plant Science·Yong CuiLiwen Jiang
Dec 19, 2019·Frontiers in Plant Science·Shino Goto-YamadaKenji Yamada
Sep 11, 2020·Journal of Cell Science·Sebastian Schuck
Apr 12, 2019·Frontiers in Plant Science·Xiaohong Zhuang, Liwen Jiang
Mar 19, 2020·Frontiers in Plant Science·Tong SuPingping Wang
Jul 1, 2020·Frontiers in Cell and Developmental Biology·Fan BuLiang Chen
Dec 24, 2018·Plant Signaling & Behavior·Sakuya Nakamura, Masanori Izumi
Dec 18, 2020·Plant Signaling & Behavior·Sakuya Nakamura, Masanori Izumi
Nov 23, 2020·The Plant Journal : for Cell and Molecular Biology·Germán RobertKohki Yoshimoto
Jan 31, 2021·Plant & Cell Physiology·Emi Ito
Aug 24, 2019·Current Opinion in Plant Biology·Jesse D Woodson
Apr 2, 2021·Plant Physiology·Jessica A S BarrosTamar Avin-Wittenberg
May 1, 2021·International Journal of Molecular Sciences·Sichul Lee, Celine Masclaux-Daubresse
May 2, 2021·Biochimica Et Biophysica Acta. General Subjects·Sakuya NakamuraMasanori Izumi
Dec 24, 2020·Plant & Cell Physiology·Sakuya NakamuraMasanori Izumi
Jun 30, 2021·Journal of Experimental Botany·Mastoureh SedaghatmehrSalma Balazadeh
Dec 16, 2021·Chemical Communications : Chem Comm·Shuhei KusanoShinya Hagihara

❮ Previous
Next ❯

Related Concepts

Related Feeds

ATG proteins

The discovery of autophagy-related ('ATG') proteins in the 1990s greatly advanced the mechanistic understanding of autophagy and clarified the fact that autophagy serves important roles in various biological processes.

Autophagosome

An autophagosome is the formation of double-membrane vesicles that involve numerous proteins and cytoplasmic components. These double-membrane vesicles are then terminated at the lysosome where they are degraded. Discover the latest research on autophagosomes here.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Autophagosome

An autophagosome is the formation of double-membrane vesicles that involve numerous proteins and cytoplasmic components. These double-membrane vesicles are then terminated at the lysosome where they are degraded. Discover the latest research on autophagosomes here.