PMID: 3214431Nov 1, 1988Paper

Selective elution of rodent glutathione S-transferases and glyoxalase I from the S-hexyglutathione-Sepharose affinity matrix

The Biochemical Journal
J D Hayes

Abstract

1. The major hepatic glutathione S-transferases (GSTs) from gerbil, guinea-pig, hamster, mouse and rat comprise Ya- (Mr 25,500-25,800), Yb- (Mr 26,100-26,400), Yc- (Mr 27,000-27,500) and Yf- (Mr 24,800) type subunits. 2. In all rodent species the GST subunits possess characteristic affinities for S-hexyglutathione-Sepharose and are eluted at distinct positions when a gradient of counter-ligand is employed to develop this affinity gel. The enzymes that bind to this matrix can be eluted, according to their subunit composition, in the order Ya-, Yc-, Yf- and Yb-containing GST; glyoxalase I, also retained by S-hexylglutathione-Sepharose, is eluted after the major GST YbYb peak. 3. Conditions are also described for the isocratic affinity elution of S-hexylglutathione-Sepharose that allow rat GST to be divided into four separate fractions (pools 1-4). A further fraction (pool 5) can be prepared from material that does not bind S-hexylglutathione-Sepharose and is obtained by chromatography on glutathione-Sepharose. 4. The sequential use of S-hexylglutathione-Sepharose and glutathione-Sepharose has facilitated the isolation of novel GSTs by enriching the various affinity-purified fractions with different subunits. This strategy allowed...Continue Reading

Citations

Jan 1, 1992·Critical Reviews in Biochemistry and Molecular Biology·S Tsuchida, K Sato
Jun 1, 1996·Protein Science : a Publication of the Protein Society·J WangR F Colman
Sep 19, 2002·Molecular Reproduction and Development·Louis Hermo, Duncan Jacks
Nov 1, 1990·Fish Physiology and Biochemistry·S G George, G Buchanan
Sep 1, 1989·Circulation Research·M W Chapleau, F M Abboud
Mar 1, 1992·Circulation Research·J L SeagardC Dean
Jan 1, 1991·Applied Biochemistry and Biotechnology·J S Dordick
Jun 1, 1996·Insect Biochemistry and Molecular Biology·V S HaritosJ T Ahokas
Nov 10, 1993·Biochimica Et Biophysica Acta·A J Hussey, J D Hayes
Apr 15, 1991·Biochimica Et Biophysica Acta·M L ClapperK D Tew
May 9, 2006·Journal of Combinatorial Chemistry·Do-Hyun KimSeung Bum Park

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