PMID: 11927014Apr 3, 2002Paper

Selective expansion of human natural killer cells from peripheral blood mononuclear cells by the cell line, HFWT

Japanese Journal of Cancer Research : Gann
Hideki HaradaT Ohno

Abstract

An anchorage-dependent Wilms tumor cell line HFWT was found to stimulate selective and remarkable expansion of human natural killer (NK) cells from human peripheral blood mononuclear cells (PBMC). After PBMC of healthy donors were cultured on irradiated HFWT cells for 10 - 21 days, the lymphocytes expanded 58- to 401-fold. This NK cell expansion required direct contact of PBMC with live, but not fixed, HFWT cells. The PBMC from an end-stage brain tumor patient also expanded 156-fold, whereas those cultured with irradiated NK-sensitive K562 grew only 30.5-fold. CD16+ CD56+ NK cells accounted for more than 70% of the population expanded on HFWT cells. No essential difference in expression of NK receptors was observed in the expanded NK cells on HFWT and K562 and without feeder cells. The expanded NK cells killed not only fresh HFWT cells but, unexpectedly, also MHC class I-expressing autologous brain tumor cells at an effector/target ratio of 4 for 24 h. These results will contribute to the development of a large-scale preparation method for human NK cells, which will aid studies of NK cell biology and possible treatment of brain tumors.

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Citations

Dec 29, 2015·Microbiology and Immunology·Shinichiro HattoriSeiji Okada
Oct 28, 2008·International Immunopharmacology·Ru ZhouJian-Ping Zuo
Apr 7, 2009·British Journal of Haematology·Hiroyuki FujisakiDario Campana
Jul 28, 2004·International Journal of Radiation Oncology, Biology, Physics·Eiichi IshikawaTadao Ohno
Nov 24, 2005·European Journal of Immunology·Hideki HaradaSeiji Okada
May 5, 2009·The Korean journal of laboratory medicine·Duck Cho, Dario Campana
Jun 14, 2013·Oncology Letters·Baskar SubramaniTerunuma Hiroshi
May 12, 2017·Frontiers in Immunology·Markus GranzinEvelyn Ullrich
May 5, 2011·Canadian Journal of Physiology and Pharmacology·T P Hamsa, Girija Kuttan

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