Selective expression of cholesterol metabolism genes in normal CD34+CD38- cells with a heterogeneous expression pattern in AML cells

Experimental Hematology
Susan D P W M PeetersEdo Vellenga

Abstract

To identify adenosine triphosphate-binding-cassette (ABC) transporters that are selectively expressed in normal and/or leukemic CD34+CD38- stem cells. Microarray experiments on murine stem cells identified 13 ABC transporters with a high expression level. Corresponding human transporters were analyzed in normal CD34+CD38- and CD34+CD38+ bone marrow cells by quantitative reverse transcriptase polymerase chain reaction. Five ABC transporters, including ABCG1, were differentially expressed with a higher expression in CD34+CD38- cells. Besides ABCG1, known to be involved in cholesterol metabolism, expression of another major cholesterol transporter (ABCA1), some cholesterol metabolism genes (3-hydroxy-3-methyl-glutaryl-CoA reductase, low-density lipoprotein receptor), and the transcription factor controlling ABCA1 and ABCG1 expression, liver-X-receptor-alpha (LXR-alpha), were assessed. All these genes were predominantly expressed in the more primitive subpopulation, indicating a high rate of cholesterol metabolism and transport. Conversely in acute myeloid leukemia (AML), a heterogeneous expression pattern was found consisting of a considerably higher expression of particularly LXR-alpha in CD34+ cells and a reverse expression patt...Continue Reading

References

Jan 1, 1995·Cytogenetics and Cell Genetics·M HöglundF Mitelman
Apr 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·J McNeishO L Francone
Aug 16, 2001·The Journal of Biological Chemistry·X FuE G Lund
Sep 14, 2002·Science·Natalia B IvanovaIhor R Lemischka
Jul 9, 2003·The Journal of Biological Chemistry·Emi KanekoMakoto Makishima
Mar 17, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Peter L GreenbergJacob M Rowe
May 4, 2004·Annals of Medicine·Albert K GroenFolkert Kuipers
Jun 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Nan WangAlan R Tall
Oct 30, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Jenny WongAndrew J Brown
Jun 1, 2005·Biochimica Et Biophysica Acta·Gerd Schmitz, Thomas Langmann

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Citations

Sep 4, 2010·Antioxidants & Redox Signaling·Hendrik GremmelsMarianne C Verhaar
Oct 12, 2010·Journal of the National Cancer Institute·Anna Maria CalcagnoSuresh V Ambudkar
Jun 26, 2010·Science·Göran K Hansson, Magnus Björkholm
Oct 31, 2009·Journal of Leukocyte Biology·Kent W Christopherson, Alan Landay
Nov 13, 2008·Annals of Hematology·Susan D P W M de Jonge-PeetersEdo Vellenga
Aug 26, 2011·Biochimica Et Biophysica Acta·Andrew J MurphyAlan R Tall
Mar 21, 2007·Critical Reviews in Oncology/hematology·Susan D P W M de Jonge-PeetersEdo Vellenga
Jul 22, 2014·Clinical and Translational Science·Kai Yin, Devendra K Agrawal
Dec 7, 2018·Frontiers in Genetics·Yaroslav R EfremovSergey S Bogachev
Apr 23, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Karen van der WeideEdo Vellenga
Sep 28, 2017·Molecular Cancer Therapeutics·Sylvain ThierryMarie Dutreix

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