Selective Expression of Flt3 within the Mouse Hematopoietic Stem Cell Compartment

International Journal of Molecular Sciences
Ciaran James MooneyGeoffrey Brown

Abstract

The fms-like tyrosine kinase 3 (Flt3) is a cell surface receptor that is expressed by various hematopoietic progenitor cells (HPC) and Flt3-activating mutations are commonly present in acute myeloid and lymphoid leukemias. These findings underscore the importance of Flt3 to steady-state and malignant hematopoiesis. In this study, the expression of Flt3 protein and Flt3 mRNA by single cells within the hematopoietic stem cell (HSC) and HPC bone marrow compartments of C57/BL6 mice was investigated using flow cytometry and the quantitative reverse transcription polymerase chain reaction. Flt3 was heterogeneously expressed by almost all of the populations studied, including long-term reconstituting HSC and short-term reconstituting HSC. The erythropoietin receptor (EpoR) and macrophage colony-stimulating factor receptor (M-CSFR) were also found to be heterogeneously expressed within the multipotent cell compartments. Co-expression of the mRNAs encoding Flt3 and EpoR rarely occurred within these compartments. Expression of both Flt3 and M-CSFR protein at the surface of single cells was more commonly observed. These results emphasize the heterogeneous nature of HSC and HPC and the new sub-populations identified are important to unders...Continue Reading

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Citations

Aug 11, 2017·Science Translational Medicine·Samuel J TaylorWallace Y Langdon
Apr 11, 2018·Immunology and Cell Biology·Geoffrey BrownRhodri Ceredig
Jul 25, 2018·International Journal of Molecular Sciences·Geoffrey BrownPanagiotis Tsapogas
Dec 22, 2019·International Journal of Molecular Sciences·Geoffrey BrownIsidro Sánchez-García
Dec 1, 2017·International Journal of Molecular Sciences·Geoffrey Brown, Ewa Marcinkowska
Jul 6, 2019·Frontiers in Cell and Developmental Biology·Geoffrey Brown, Rhodri Ceredig
Apr 3, 2020·International Journal of Molecular Sciences·Geoffrey Brown
Mar 28, 2020·International Journal of Molecular Sciences·Geoffrey BrownIsidro Sánchez-García
May 26, 2017·International Journal of Molecular Sciences·Panagiotis TsapogasAntonius Rolink
Oct 28, 2020·The Journal of Clinical Investigation·Alhomidi AlmotiriNeil P Rodrigues
Jun 3, 2021·Bioengineering·Geoffrey Brown
Jun 28, 2020·Molecular Therapy : the Journal of the American Society of Gene Therapy·Cesar SommerBarbra J Sasu

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Methods Mentioned

BETA
flow cytometry
MDS
imaging techniques
FCS

Software Mentioned

Summit

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