PMID: 11897999Mar 19, 2002Paper

Selective expression of nuclear factor of activated T cells 2/c1 in human basophils: evidence for involvement in IgE-mediated IL-4 generation

The Journal of Allergy and Clinical Immunology
John T SchroederV Casolaro

Abstract

The nuclear factor of activated T cells (NFAT) family of transcription factors plays a key role in rapidly inducing IL4 gene expression in effector T cells. Because human basophils secrete high levels of IL-4, we have examined whether specific NFAT species are expressed in these cells and whether Fc(epsilon)RI-mediated activation affects their subcellular localization and transcriptional function. Intracellular NFAT protein was identified by using 2-color flow cytometry; gene expression was done with RT-PCR. Subcellular localization of NFAT was assessed by means of Western blotting. Electrophoretic mobility shift assays assessed NFAT involvement in IL-4 transcription. Basophils constitutively expressed high levels of NFAT2. In contrast, NFAT1 (NFATp), which is found in most leukocytes, was not seen in basophils. Low-level staining for NFAT4 was detected but was variably expressed among donor cells. Likewise, NFAT2 mRNA was constitutively expressed in basophils, and message for NFAT4 was seen in 3 of 5 preparations, whereas that for NFAT1 was found in only 1 of 5 preparations. NFAT2 protein accumulated in the nuclei of basophils activated for 1 hour with anti-IgE, and this was inhibited with the addition of FK506. A protein-DNA ...Continue Reading

Citations

May 23, 2012·Blood·Jan FricPaola Ricciardi-Castagnoli
Jun 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wai-Hang Leung, Silvia Bolland
Mar 28, 2013·Computational and Mathematical Methods in Medicine·Yu-wen LvHai-tao Lv
Jun 21, 2011·Immunological Reviews·John T Schroeder
Oct 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·John T SchroederMark C Liu
Mar 25, 2015·Molecular Immunology·Tsukasa UgajinToshio Hirano

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