Selective fluorescent labeling of S-nitrosothiols (S-FLOS): a novel method for studying S-nitrosation.

Nitric Oxide : Biology and Chemistry
Lakshmi SanthanamRobert N Cole

Abstract

Protein S-nitrosation is a reversible post-translation modification critical for redox-sensitive cell signaling that is typically studied using the Biotin Switch method. This method and subsequent modifications usually require avidin binding or Western blot analysis to detect biotin labeled proteins. We describe here a modification of the Biotin Switch assay that eliminates the need for Western blot or avidin enrichment protocols and allows direct comparison of the S-nitrosation state proteins from two different samples in the same gel lane or on the same 2D gel. This S-FLOS method offers detection, identification and quantification of S-nitrosated proteins, with the potential for site-specific identification of nitrosation events.

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Citations

May 3, 2011·Molecular and Cellular Biochemistry·Jessilyn DunnLakshmi Santhanam
Jul 4, 2012·Antioxidants & Redox Signaling·Pablo Hernansanz-AgustínAntonio Martínez-Ruiz
Nov 20, 2012·Antioxidants & Redox Signaling·Alicia M EvangelistaElizabeth Murphy
Jan 14, 2011·Biology of Reproduction·Hong-hai ZhangDong-bao Chen
Oct 30, 2012·Circulation Research·Christopher I Murray, Jennifer E Van Eyk
Nov 7, 2015·Journal of Proteome Research·Zhe QuZezong Gu
Mar 22, 2013·Chemical Reviews·Candice E Paulsen, Kate S Carroll
Jul 22, 2009·Journal of Proteomics·Christian Lindermayr, Jörg Durner
May 15, 2009·Free Radical Biology & Medicine·Clare L HawkinsMichael J Davies
Nov 3, 2010·Current Opinion in Chemical Biology·Hua Wang, Ming Xian
Feb 24, 2015·Journal of the American Society for Mass Spectrometry·William O SladeLeslie M Hicks
Dec 16, 2017·Chemical Society Reviews·Lisa J AlcockJustin M Chalker
Sep 26, 2017·Chemical Communications : Chem Comm·Caihong ZhangMing Xian

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