Selective gene delivery in dendritic cells with mannosylated and histidylated lipopolyplexes

Journal of Drug Targeting
Federico PerchePatrick Midoux

Abstract

We report for the first time preparation of mannosylated and histidylated lipopolyplexes (Man-LPD100) with uptake and transfection selectivity for dendritic cells (DCs). Man-LPD100 were prepared by addition of mannosylated and histidylated liposomes (Man-Lip100) on preformed PEGylated histidylated polylysine/DNA polyplexes. Man-Lip100 comprised a cationic [O,O-dioleyl-N-(3N-(N-methylimidazolium iodide)propylene) phosphoramidate)] lipid, a neutral [O,O-dioleyl-N-histamine Phosphoramidate] co-lipid and β-D-mannopyranosyl-N-dodecylhexadecanamide (Man-lipid). At the best, Man-Lip100 containing 11 mol % Man-lipid was obtained. We found that dialysis of liposomes completely abolished cytotoxicity. We showed that the uptake of Man(11)-LPD100 by the murine DC line (DC2.4 cells) was at least 10-fold higher than that of Lac(6)-LPD100. A confocal microscopy study with DC2.4 cells expressing Rab5-EGFP or Rab7-EGFP, revealed that DNA uptake occurred through clathrin-mediated endocytosis. The transfection of DC2.4 cells with Man(11)-LPD100 containing DNA encoding luciferase gene gave luciferase activity two to three times higher (9 × 10(5) RLU/mg protein) than with non-mannosylated LPD100. In contrast to the latter, it was inhibited by 90% i...Continue Reading

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Citations

Jun 30, 2012·International Journal of Nanomedicine·Xun SunZhirong Zhang
Jan 26, 2013·Biotechnology Advances·Virendra Gajbhiye, Shaoqin Gong
Jul 9, 2014·Molecular Therapy. Nucleic Acids·Kenneth C McCulloughNicolas Ruggli
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Jun 19, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Mehdi RezaeeBizhan Malaekeh-Nikouei
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Jun 7, 2014·Nanoscale·Kyle K L PhuaKam W Leong
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Sep 7, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Mathieu BerchelPatrick Midoux
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Jan 23, 2021·Vaccines·Michael D BuschmannDrew Weissman
Oct 7, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Wenxing GuZhiyuan Zhong
Aug 28, 2020·Molecular Pharmaceutics·Satoshi UchidaHoracio Cabral
Aug 28, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Viorel SimionPatrick Baril
Jul 3, 2021·Pharmaceutics·Christophe DeleheddeFederico Perche
Aug 28, 2021·International Journal of Molecular Sciences·Shabnam TarvirdipourCornelia G Palivan

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