PMID: 6407003May 1, 1983Paper

Selective gene expression induced by ecdysterone in cultured fat bodies of Drosophila

Proceedings of the National Academy of Sciences of the United States of America
Y Nakanishi, A Garen

Abstract

Expression of the LSP-2 and P1 genes was induced in cultured fat bodies of Drosophila third-instar larvae by supplementing the culture medium with ecdysterone. The fat bodies were isolated from ecdysterone-deficient larvae of the temperature-sensitive mutant ecd1, which were shifted from the permissive to the restrictive temperature either at the beginning of the third instar for the detection of LSP-2 induction or several hours later for the detection of P1 induction. During normal larval development, the LSP-2 gene is expressed before the P1 gene, and this order is also observed in the cultured fat bodies. Induction was demonstrated by increased amounts of LSP-2 and P1 transcripts in the ecdysterone-supplemented fat bodies. The amount of P1 transcript was determined by two methods: one involved measuring the hybridization of a labeled P1 DNA probe to total fat body RNA; the other involved labeling the newly synthesized RNA in nuclei isolated from cultured fat bodies and measuring the hybridization of the labeled RNA to P1 DNA. Only the second method was used for the LSP-2 transcript because the earlier expression of the LSP-2 gene results in a measurable accumulation of the transcript in the fat bodies before ecdysterone supp...Continue Reading

References

Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J A LepesantA Garen
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J L ManleyM L Gefter
Oct 1, 1977·Developmental Biology·R B HodgettsJ D O'Connor
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Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·H Gronemeyer, O Pongs

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Citations

Jan 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M Lehmann
Jan 1, 1993·Developmental Genetics·V C HenrichL I Gilbert
Nov 1, 1990·Developmental Biology·H BenesD W Spivey
Jul 30, 1990·Biochimica Et Biophysica Acta·M TamaruY Nakanishi
Apr 1, 1997·European Journal of Biochemistry·S Mousseron-GrallJ A Lepesant
Jul 20, 1990·Journal of Molecular Biology·F MaschatJ A Lepesant
Jan 1, 1990·Archives of Insect Biochemistry and Physiology·G Sommé-MartinR Lafont
Dec 1, 1990·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C S Thummel
Oct 1, 1987·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Aparna RayAlapati Krishna Kumaran
Jan 1, 1989·Developmental Genetics·J DeutschL Rat

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