PMID: 8944333Jan 1, 1996Paper

Selective growth of freshly isolated human breast epithelial cells cultured at low concentrations in the presence or absence of bone marrow cells

Breast Cancer Research and Treatment
Joanne T EmermanConnie J Eaves

Abstract

In this study, we show that conditions previously found to promote the selective growth of human breast epithelial cells (HBEC) in serum-free primary cultures established from normal or malignant tissue can be extended to cultures initiated at low seeding densities (< 5000 cells/cm2). The epithelial nature of the cells produced was documented by their positive staining with antibodies specific for keratins 8, 14, and 18, and 2 antibodies that recognize epithelial-specific antigens (Ber-EP4 and HB8630). HBEC growth was not affected, either positively or negatively, by the use of a medium containing a combination of fetal calf and horse serum, which promotes the growth of many types of stromal cells and associated hematopoietic precursors, or by the inclusion in the initial cell suspension of marrow cells at HBEC to marrow cell ratios typical of bone marrow samples from patients with metastatic breast cancer. The presence of fibroblast feeders from a variety of sources enhanced the growth of HBEC to different degrees. In cultures initiated with low numbers of cells obtained from samples of breast carcinoma, HBEC growth was generally reduced by comparison to cultures of normal HBEC. With the detection methods used, it was not poss...Continue Reading

References

Jun 15, 1978·The New England Journal of Medicine·S E SalmonT E Moon
Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·O W PetersenM J Bissell
Mar 1, 1990·Journal of Clinical Pathology·U LatzaH Stein
Jan 1, 1991·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J L MansiR C Coombes
Jan 1, 1991·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·D P DearnaleyJ P Sloane
Oct 1, 1991·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·R J CoteM P Osborne
Dec 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J T Emerman, D A Wilkinson
Oct 1, 1989·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·G Bonadonna
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·V Band, R Sager
Jan 1, 1984·Recent Results in Cancer Research. Fortschritte Der Krebsforschung. Progrès Dans Les Recherches Sur Le Cancer·C Cillo, N Odartchenko
Jan 1, 1981·Journal of Surgical Oncology·S LanM R Stampfer
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·G SauvageauR K Humphries
Jun 1, 1993·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·W P PetersE Shpall

❮ Previous
Next ❯

Citations

Apr 30, 2013·Journal of Mammary Gland Biology and Neoplasia·Maisam MakaremConnie J Eaves
Nov 20, 1998·Breast Cancer Research and Treatment·N L CooperJ T Emerman
Oct 9, 2003·Breast Cancer Research and Treatment·Ladislav TomesPeter H Watson
Mar 21, 1998·Journal of Hematotherapy·A A Ross
Sep 16, 2009·Journal of Experimental & Clinical Cancer Research : CR·Ralf Hass, Catharina Bertram
Sep 24, 1998·Differentiation; Research in Biological Diversity·J StinglJ T Emerman
Nov 6, 2007·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Véronique MargueriteMarc Merten
Sep 16, 2004·Springer Seminars in Immunopathology·Yago NietoElizabeth J Shpall

❮ Previous
Next ❯

Related Concepts

Related Feeds

Breast Cancer: Chemo-Resistance

Some cancers are difficult to treat and aggressive including the "triple-negative" breast cancer. This type of cancer is chemoresistant even before chemotherapy begins. Here are the latest discoveries chemo-resistance in breast cancer.