Selective Hydrolysis of Terminal Glycosidic Bond in α-1-Acid Glycoprotein Promoted by Keggin and Wells-Dawson Type Heteropolyacids.

Chemistry : a European Journal
Laura S Van RompuyTatjana N Parac-Vogt

Abstract

The reactivity of a range of Keggin and Wells-Dawson type heteropolyacids (HPAs): H3 PW12 O40 H4 SiW12 O40 , H3 PMo12 O40 , K6 P2 W18 O62 , and NaH2 W12 O4 , towards the heavily glycosylated α-1-acid glycoprotein (AGP) is reported. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains. The 1 H NMR spectroscopy confirmed that the released sialic acids preserve intact structure upon their excision from the protein, which makes the reported method suitable for the analysis of sialic acid modifications which play an important role in numerous biological processes. The presence of other sugars was not detected by 1 H NMR and HPAEC-PAD, suggesting that HPAs hydrolyze only the terminal glycosidic bond in the glycoprotein, resulting in the selective release of sialic acid from AGP. The kinetic results have shown that under equal temperature and pH conditions, the hydroly...Continue Reading

References

Mar 1, 1969·The Journal of Cell Biology·L Silverman, D Glick
Feb 1, 1982·European Journal of Biochemistry·J HaverkampR Schauer
Nov 4, 2000·Biochimica Et Biophysica Acta·T FournierD Porquet
May 18, 2001·Journal of Chromatography. a·C J ShawB Xiao
Jun 26, 2001·Glycoconjugate Journal·R Schauer
Jan 31, 2002·Analytical Chemistry·A LiR J Fisher
Nov 27, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Fotini N Lamari, Nikos K Karamanos
Jun 3, 2004·Electrophoresis·Giovanni CandianoPier Giorgio Righetti
Jun 16, 2005·Current Opinion in Pharmacology·Paul R Crocker
Oct 13, 2006·Nature Biotechnology·Gary Walsh, Roy Jefferis
Mar 24, 2007·Nature Reviews. Immunology·Paul R CrockerAjit Varki
Mar 21, 2008·Journal of Pharmaceutical Sciences·Andria L Skinner, Jennifer S Laurence
Feb 14, 2009·Chemical Communications : Chem Comm·Nele SteensTatjana N Parac-Vogt
Aug 1, 2009·Journal of Pharmaceutical Sciences·Yijia JiangLinda Narhi
Nov 6, 2009·Glycoconjugate Journal·Elizabeth Higgins
Feb 5, 2010·Angewandte Chemie·De-Liang LongLeroy Cronin
Sep 11, 2010·Nature Biotechnology·Gary Walsh
Nov 20, 2010·Dalton Transactions : an International Journal of Inorganic Chemistry·Phuong Hien HoTatjana N Parac-Vogt
Nov 8, 2011·Journal of Chemical Education·Adam W BarbJames H Prestegard
Apr 25, 2012·Annals of the New York Academy of Sciences·Ajit Varki, Pascal Gagneux
Aug 2, 2012·Chemical Society Reviews·Yu-Fei Song, Ryo Tsunashima
Aug 30, 2012·Inorganic Chemistry·Gregory Absillis, Tatjana N Parac-Vogt
Sep 8, 2012·Inorganic Chemistry·Tzvetan MihaylovKristine Pierloot
Jun 22, 2013·Dalton Transactions : an International Journal of Inorganic Chemistry·Hong Giang T LyTatjana N Parac-Vogt
Sep 26, 2013·Physical Chemistry Chemical Physics : PCCP·Vincent GoovaertsTatjana N Parac-Vogt
Jul 16, 2014·Biochimica Et Biophysica Acta·Christian BüllGosse J Adema
Sep 13, 2014·Dalton Transactions : an International Journal of Inorganic Chemistry·Annelies SapTatjana N Parac-Vogt
Feb 26, 2015·Journal of Physiology and Biochemistry·Zhumin LuoDing-Feng Su
May 13, 2015·Chemical Reviews·Sa-Sa Wang, Guo-Yu Yang
Nov 26, 2015·MAbs·Lei ZhangBaolin Zhang
Dec 3, 2016·Nucleic Acids Research·UNKNOWN The UniProt Consortium
Dec 12, 2018·Current Opinion in Biotechnology·Laura S Van Rompuy, Tatjana N Parac-Vogt

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