Selective Immobilization of Fluorescent Proteins for the Fabrication of Photoactive Materials

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Ana I Benítez-MateosFernando López-Gallego

Abstract

The immobilization of fluorescent proteins is a key technology enabling to fabricate a new generation of photoactive materials with potential technological applications. Herein we have exploited superfolder green (sGFP) and red (RFP) fluorescent proteins expressed with different polypeptide tags. We fused these fluorescent proteins to His-tags to immobilize them on graphene 3D hydrogels, and Cys-tags to immobilize them on porous microparticles activated with either epoxy or disulfide groups and with Lys-tags to immobilize them on upconverting nanoparticles functionalized with carboxylic groups. Genetically programming sGFP and RFP with Cys-tag and His-tag, respectively, allowed tuning the protein spatial organization either across the porous structure of two microbeads with different functional groups (agarose-based materials activated with metal chelates and epoxy-methacrylate materials) or across the surface of a single microbead functionalized with both metal-chelates and disulfide groups. By using different polypeptide tags, we can control the attachment chemistry but also the localization of the fluorescent proteins across the material surfaces. The resulting photoactive material formed by His-RFP immobilized on graphene h...Continue Reading

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Citations

Dec 22, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Roberto Fernandez-Lafuente
Jul 29, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yuhong QianZhuang Li

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Methods Mentioned

BETA
FRET
biosensors
chips
Scanning Microscopy
fluorescence microscopy
Electrophoresis

Software Mentioned

ImageJ
FIJI
ZEISS
ZEN

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