PMID: 11607223Oct 15, 1991Paper

Selective induction of gene expression and second-messenger accumulation in Dictyostelium discoideum by the partial chemotactic antagonist 8-p-chlorophenylthioadenosine 3',5'-cyclic monophosphate

Proceedings of the National Academy of Sciences of the United States of America
D J PetersP Schaap

Abstract

During development of the cellular slime mold Dictyostelium discoideum, cAMP induces chemotaxis and expression of different classes of genes by means of interaction with surface cAMP receptors. We describe a cAMP derivative, 8-p-chlorophenylthioadenosine 3',5'-cyclic monophosphate (8-CPT-cAMP), which inhibits cAMP-induced chemotaxis at low concentrations but induces chemotaxis at supersaturating concentrations. This compound, moreover, selectively activates expression of aggregative genes but not of postaggregative genes. 8-CPT-cAMP induces normal cGMP and cAMP accumulation but in contrast to cAMP, which increases inositol 1,4,5-trisphosphate levels, 8-CPT-cAMP decreases inositol 1,4,5-trisphosphate levels. The derivative induces reduced activation of guanine nucleotide regulatory proteins, which may cause its defective activation of inositol 1,4,5-trisphosphate production. Our data suggest that disruption of inositolphospholipid signaling impairs chemotaxis and expression of a subclass of cAMP-regulated genes.

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Citations

Dec 18, 1995·Experientia·C D ReymondJ G Williams
May 23, 2007·The Journal of Cell Biology·Ineke Keizer-GunninkPeter J M Van Haastert
Jul 29, 2008·Ageing Research Reviews·Vanessa C McMainsAlan R Kimmel
Jul 15, 1992·Biochemical and Biophysical Research Communications·R Valkema, P J Van Haastert
Sep 1, 1994·Microbiological Reviews·J D Gross
Jul 11, 1997·Science·B Wang, A Kuspa

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