PMID: 8975877Dec 1, 1996Paper

Selective induction of peripheral and mucosal endothelial cell addressins with peripheral lymph nodes and Peyer's patch cell-conditioned media

Journal of Leukocyte Biology
V DenisC Kieda

Abstract

Vascular endothelial cell addressins play an important role in lymphocyte homing in secondary lymphoid organs and in chronic inflammatory areas. A SV40 large T antigen-immortalized cell line from peripheral lymph nodes, HECa1O [Bizouarne et al., 1993a], was used to characterize the location of addressins with regard to environmental factors and cytokines. For this purpose, two monoclonal antibodies, MECA 79 and MECA 367, specific for peripheral lymph node vascular addressin and for mucosal addressin (Peyer's patches), respectively, were bound to unstimulated HECa1O cells. Both mucosal and peripheral addressins were detected inside the cells and in cellular extracts of the resting cells. On the cell surface, both addressins could be evidenced on the same cells at a moderate level of expression. They partly mediate the EL4/EL4IL2 lymphoma cells' adhesion to HECa1O cells. Supernatants of cultured peripheral lymph node or Peyers' patch cells induced expression of MECA 79 or MECA 367 antigens, respectively, on the surface of HECa1O cells. Interleukins, IL-7, IL-3, and IL-8, induced the cell-surface appearance of MECA 79 but not of MECA 367 antigen. Therefore, the same cell type synthesizes both antigens, but the expression of these ...Continue Reading

Citations

Jul 9, 2009·Archivum Immunologiae Et Therapiae Experimentalis·Maud RacapéSophie Brouard
Feb 8, 2003·Endothelium : Journal of Endothelial Cell Research·C KiedaD Duś
Dec 3, 1999·Immunological Reviews·P BrandtzaegT Yamanaka
Mar 9, 2013·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Claudine KiedaClaude Nicolau
Jan 13, 2021·International Journal of Molecular Sciences·Aleksandra MajewskaClaudine Kieda

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