Selective inhibition of antigen-induced "step one" in cytotoxic T lymphocytes by anti-Lyt-2 antibodies

European Journal of Immunology
M Gullberg, E L Larsson

Abstract

Stimulation of mixed lymphocyte cultures with ultraviolet (UV)-irradiated stimulator cells leads to selective activation of Lyt-2+ cytotoxic T lymphocyte precursors (CTL-P) to acquire interleukin 2 (IL2) reactivity: (a) no proliferative response, nor IL2 production was observed in these cultures; (b) upon addition of preformed IL2-conditioned media (IL2-CM), a vigorous proliferative response was observed; (c) the IL2-dependent proliferation was reduced by approximately 90% after depletion of Lyt-2+ responder cells; and (d) specific CTL were generated upon the initial triggering by UV-irradiated stimulators and IL2 expansion. This system therefore provides suitable conditions for studying the role of Lyt-2 antigens in initial, antigen-specific induction of CTL-P, as well as at their effector phase. It is shown herein that monoclonal anti-Lyt-2 antibodies inhibit antigen-specific acquisition of IL2 reactivity by CTL-P, which results in 85% reduction of the proliferative response mediated by IL2-CM. CTL-P which have been induced by antigen to IL2 reactivity, prior to addition of anti-Lyt-2 antibodies, however, are not affected in their IL2-dependent proliferation. Furthermore, both the specific and lectin-dependent cytolytic activ...Continue Reading

References

Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·E NakayamaL J Old
Aug 1, 1979·European Journal of Immunology·A CoutinhoJ Andersson
Jan 1, 1977·Immunological Reviews·K J Lafferty, J Woolnough
Jul 19, 1979·Nature·E L Larsson, A Coutinho
Oct 1, 1973·European Journal of Immunology·M H JuliusL A Herzenberg
Mar 1, 1981·The Journal of Experimental Medicine·D P DialynasF W Fitch
Feb 1, 1980·European Journal of Immunology·E L Larsson, A Coutinho
Sep 1, 1980·The Journal of Experimental Medicine·N HollanderI L Weissman

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Citations

Aug 1, 1984·Biulleten' eksperimental'noĭ biologii i meditsiny·A V FilatovB D Brondz
May 1, 1984·European Journal of Immunology·E L Larsson, A Coutinho

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