Selective inhibition of cytokine-induced lysozyme activity by tetanus toxin in the GG2EE macrophage cell line.

Infection and Immunity
L PitzurraE Blasi

Abstract

This study was designed to evaluate the effects of tetanus toxin (TT) on lysozyme (LZM) activity by the GG2EE macrophage cell line. GG2EE cells spontaneously produced low amounts of LZM, which were mostly secreted into the culture medium. Upon treatment with various cytokines, GG2EE cells exhibited altered LZM activity. In particular, exposure of GG2EE cells to alpha/beta interferon (IFN-alpha/beta) reduced LZM activity, as opposed to treatment with gamma interferon (IFN-gamma) or colony-stimulating factor 1, which potentiated LZM activity. Spontaneous LZM activity of GG2EE cells was not susceptible to TT action; in contrast, when IFN-gamma- or colony-stimulating factor 1-susceptible cells were treated with TT, a significant reduction on LZM activity was observed. The TT inhibitory effect was dose dependent and manifested only after a 6-h incubation of GG2EE cells with TT. Treatment of GG2EE cells with heat-inactivated TT as well as Ibc- and B-IIb-TT-derived fragments was found to be ineffective, while pretreatment with B-IIb- but not with Ibc-TT-derived fragment abrogated the TT effect. Overall, these data indicate the existence of a specific TT-GG2EE cell interaction, leading to selective inhibition of cytokine-induced LZM ac...Continue Reading

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Citations

Jun 10, 2003·Journal of Applied Toxicology : JAT·Brian M Paddle
Sep 27, 2014·Mediators of Inflammation·Tim KlüterDeike Varoga
Sep 1, 1990·Scandinavian Journal of Immunology·E BlasiF Bistoni
Apr 23, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R PellizzariC Montecucco
Dec 1, 1993·FEMS Immunology and Medical Microbiology·L PitzurraE Blasi
Apr 4, 2000·Physiological Reviews·G SchiavoC Montecucco
May 1, 1995·Progress in Neurobiology·G Ahnert-Hilger, H Bigalke

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