Selective inhibition of inward rectifier K+ channels (Kir2.1 or Kir2.2) abolishes protection by ischemic preconditioning in rabbit ventricular cardiomyocytes

Circulation Research
Roberto J DiazGregory J Wilson

Abstract

Volume regulatory Cl- channels are key regulators of ischemic preconditioning (IPC). Because Cl- efflux must be balanced by an efflux of cations to maintain cell membrane electroneutrality during volume regulation, we hypothesize that I(K1) channels may play a role in IPC. We subjected cultured cardiomyocytes to 60-minute simulated ischemia (SI) followed by 60-minute of simulated reperfusion (SR) and assessed percent cell death using trypan blue staining. Ischemic preconditioning (10-minute SI/10-minute SR) significantly (P<0.0001) reduced the percent cell death in nontransfected cardiomyocytes [IPC(CM) 18.0+/-2.1% versus control (C(CM)) 48.3+/-1.0%]. IPC protection was not altered by overexpression of the reporter gene (enhanced green fluorescent protein, EGFP). However, overexpression of dominant-negative Kir2.1 or Kir2.2 genes using adenoviruses (AdEGFPKir2.1DN or AdEGFPKir2.2DN) encoding the reporter gene EGFP prevented IPC protection [both IPC(CM)+AdEGFPKir2.1DN 45.8+/-2.3% (mean+/-SEM) and IPC(CM)+AdEGFPKir2.2DN 47.9+/-1.4% versus IPC(CM); P<0.0001] in cultured cardiomyocytes (n=8 hearts). Transfection of cardiomyocytes with AdEGFPKir2.1DN or AdEGFPKir2.2DN did not affect cell death in control (nonpreconditioned) cardiomy...Continue Reading

References

Jun 1, 1995·Current Opinion in Neurobiology·C A DoupnikH A Lester
Feb 1, 1996·Pflügers Archiv : European journal of physiology·M Takano, F M Ashcroft
Jan 1, 1997·Journal of Molecular and Cellular Cardiology·R J Diaz, G J Wilson
Jan 1, 1997·Annual Review of Physiology·C G Nichols, A N Lopatin
Jan 10, 1998·The Journal of Physiology·L Y Jan, Y N Jan
Jul 19, 2002·American Journal of Physiology. Heart and Circulatory Physiology·R RajashreeP A Hofmann
Aug 6, 2002·Cardiovascular Research·Michelle BatthishGregory J Wilson
Feb 25, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Richard J GuminaAndre Terzic
Mar 8, 2003·Journal of Molecular and Cellular Cardiology·Roberto J DiazGregory J Wilson
Aug 14, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Garrett J Gross, Jason N Peart

❮ Previous
Next ❯

Citations

Apr 17, 2008·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Zhiguo WangBaofeng Yang
Apr 23, 2013·Naunyn-Schmiedeberg's Archives of Pharmacology·Wei HuangHong-Li Sun
Feb 19, 2008·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Jingdong LiTianpen Cui
Jul 11, 2008·Cardiovascular Research·Baofeng YangZhiguo Wang
Mar 14, 2013·Cardiovascular Research·Roberto J DiazGregory J Wilson
Jul 11, 2006·American Journal of Physiology. Cell Physiology·Zhu-Zhong MeiLin-Hua Jiang
Sep 6, 2007·The Journal of Clinical Investigation·Eva van Rooij, Eric N Olson
Dec 19, 2008·Journal of Cell Science·Jian-Fu ChenDa-Zhi Wang
Oct 17, 2012·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Jiang ZhuLixin Liu
Aug 26, 2009·Journal of Molecular and Cellular Cardiology·Justus M B Anumonwo, Anatoli N Lopatin
Mar 15, 2011·The FEBS Journal·Koh OnoJiahuai Han
Feb 20, 2007·Biophysical Journal·K Andrew MacCannellWayne R Giles
Mar 30, 2010·Heart Failure Clinics·Roy BeinartDavid Milan
Aug 15, 2012·International Journal of Cardiology·Hongli ShanZhimin Du
Jun 23, 2015·Cardiovascular Drugs and Therapy·Xinran LiSuhua Yan
Apr 20, 2011·Biochemical and Biophysical Research Communications·Ioana AlesutanFlorian Lang
Jul 30, 2014·Journal of Cardiovascular Pharmacology·Bo-Wei Wu, Ji-Min Cao
Sep 2, 2009·Biological & Pharmaceutical Bulletin·Xuelian LiBaofeng Yang
Apr 25, 2014·American Journal of Physiology. Cell Physiology·Roberto J DiazGregory J Wilson
Sep 4, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Jianqing ZhangMary O Gray
Jan 1, 2014·SAGE Open Medicine·Upinder KaurB L Pandey
Apr 8, 2021·American Journal of Physiology. Cell Physiology·C David Weaver, Jerod S Denton

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.