Selective inhibition of polysialyltransferase ST8SiaII by unnatural sialic acids

Experimental Cell Research
Rüdiger HorstkorteRita Gerardy-Schahn

Abstract

Polysialic acid (polySia) is a unique and highly regulated posttranslational modification of the neural cell adhesion molecule (NCAM). The presence of polySia affects NCAM-dependent cell adhesion and plays an important role during brain development, neural regeneration and plastic processes including learning and memory. Polysialylated NCAM is expressed on several neuroendocrine tumors of high malignancy and correlates with poor prognosis. Two closely related enzymes, the polysialyltransferases ST8SiaII and ST8SiaIV, catalyze the biosynthesis of polySia. However, the impact of each enzyme in NCAM polysialylation is not understood. Here, we describe the selective cell-based in vitro inhibition of ST8SiaII using synthetic sialic acid precursors. We provide evidence for different substrate affinities of ST8SiaII and ST8SiaIV. These data open the possibility to study the individual role of the two enzymes during various aspects of brain development and function and in tumorigenesis.

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Citations

Aug 26, 2014·PloS One·Vinayaga S GnanapragassamRüdiger Horstkorte
May 3, 2008·Chemical Reviews·Heather E Murrey, Linda C Hsieh-Wilson
May 5, 2009·The Journal of Biological Chemistry·Thomas J MorleyStephen G Withers
Aug 14, 2009·Glycobiology·Jian DuKevin J Yarema
Nov 10, 2011·Cellular and Molecular Life Sciences : CMLS·Johannes VogtRüdiger Horstkorte
Aug 3, 2005·Nature Reviews. Cancer·Mark M Fuster, Jeffrey D Esko
Feb 28, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nils B BayerSebastian P Galuska
Nov 7, 2014·Critical Reviews in Biochemistry and Molecular Biology·Karen J ColleyChihiro Sato
Jan 8, 2015·Proceedings of the National Academy of Sciences of the United States of America·Kyungtae KangInsung S Choi
Nov 18, 2015·Chembiochem : a European Journal of Chemical Biology·Bo ChengXing Chen
Nov 18, 2006·Biochimica Et Biophysica Acta·Daniel GagiannisRüdiger Horstkorte
May 7, 2013·American Journal of Human Genetics·Ida J HatoumLee M Kaplan
Aug 21, 2013·PloS One·Yousef M J Al-SarairehRobert A Falconer
Jul 21, 2016·Angewandte Chemie·Paul R WratilWerner Reutter
Sep 30, 2016·Medicinal Research Reviews·Rémi Szabo, Danielle Skropeta
May 5, 2017·Chembiochem : a European Journal of Chemical Biology·Jörg EhritRita Gerardy-Schahn
Feb 20, 2007·Molecular BioSystems·Christopher T CampbellKevin J Yarema
Jun 11, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Manimozhi NagasundaramVinayaga Srinivasan Gnanapragassam
Oct 26, 2018·Emerging Topics in Life Sciences·Surbhi GoswamiSrinivasa-Gopalan Sampathkumar

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