Selective injection system into hippocampus CA1 via monitored theta oscillation

PloS One
Jyoji TsutajimaKogo Takamiya

Abstract

Methods of cell biology and electrophysiology using dissociated primary cultured neurons allow in vitro study of molecular functions; however, analysis of intact neuronal circuitry is often preferable. To investigate exogenous genes, viral vectors are most commonly injected using a pipette that is inserted from the top of the cortex. Although there are few reports that describe the success rate of injection in detail, it is sometimes difficult to locate the pipette tip accurately within the CA1 pyramidal cell layer because the pyramidal layer is only 0.1 mm thick. In the present study, we have developed a system to inject viral vectors accurately into the mouse hippocampal CA1 pyramidal cell layer using a stereotaxic injection system with simultaneous electrophysiological monitoring of theta oscillation. The pipette tip was positioned reliably based on integrated values of the theta oscillation in the hippocampal CA1 pyramidal cell layer. This approach allows accurate injection of solutions and provides an efficient method of gene transfer using viral vectors into the hippocampus, which can be a useful tool for studies involving the molecular mechanisms of neuronal functions.

References

Jan 1, 1989·British Journal of Clinical Pharmacology·B S Meldrum
Jan 7, 1993·Nature·T V Bliss, G L Collingridge
Sep 18, 1999·Science·R C Malenka, R A Nicoll
Jun 9, 2000·Annual Review of Neuroscience·S J MartinR G Morris
Mar 10, 2001·Molecular Therapy : the Journal of the American Society of Gene Therapy·M Y MastakovM J During
Feb 8, 2002·Neuron·György Buzsáki
Jun 8, 2002·Annual Review of Neuroscience·Roberto Malinow, Robert C Malenka
Oct 9, 2002·Current Opinion in Neurobiology·Philip Washbourne, A Kimberley McAllister
Oct 24, 2002·Trends in Neurosciences·Reed C Carroll, R Suzanne Zukin
Oct 24, 2002·Trends in Neurosciences·Insuk Song, Richard L Huganir
Mar 12, 2003·Trends in Biotechnology·Kenneth Lundstrom
May 3, 2003·Nature Reviews. Genetics·Clare E ThomasMark A Kay
Aug 9, 2003·Science·Thomas SeidenbecherHans-Christian Pape
Jan 13, 2004·Physiological Reviews·M A Lynch
Mar 12, 2004·Biological Psychiatry·Joe Guillaume Pelletier, Denis Paré
Jan 26, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Corinna BurgerRonald J Mandel
Feb 26, 2005·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Markus M LindroosIrma E Holopainen
Jul 12, 2005·Nature Neuroscience·Chong-Hyun KimRichard Huganir
Jan 25, 2007·Nature Reviews. Molecular Cell Biology·Christian Haass, Dennis J Selkoe
Apr 6, 2007·The European Journal of Neuroscience·Rajeevan T NarayananHans-Christian Pape
Feb 17, 2009·Neuron·Helmut W Kessels, Roberto Malinow
Mar 21, 2009·Science·Viviana GradinaruKarl Deisseroth
May 1, 2009·Hippocampus·Loren M DevitoHoward Eichenbaum
Jan 5, 2010·Nature Reviews. Neuroscience·Susanne Diekelmann, Jan Born
May 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Yuichi MakinoRichard L Huganir

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Citations

Sep 15, 2015·Nature Biotechnology·Taeyoon KyungWon Do Heo

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Software Mentioned

MiniAnalysis
pClamp10
R
StatPlus

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